Headspace solid phase micro extraction gas chromatographic determination of fenthion in human serum

• Published in: International journal of molecular sciences Vol. 9; no. 5; pp. 906 - 913
• Main Authors: Kasiotis, Konstantinos M, Souki, Helen, Tsakirakis, Angelos N, Carageorgiou, Haris, Theotokatos, Spiridon A, Haroutounian, Serkos A, Machera, Kyriaki
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-05-2008; Molecular Diversity Preservation International (MDPI)
• Subjects: Chromatography; fenthion; GC/MS; headspace; Insecticide residue; Insecticides; Metabolites; Methods; olive; SPME; Greece; Athens Greece; fenthion; Insecticide residue; SPME; olive; headspace; metabolites; GC/MS
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms9050906

A simple and effective analytical procedure was developed for the determination of fenthion residues in human serum samples. The sample treatment was performed using the headspace solid-phase micro extraction with polyacrylate fiber, which has the advantage to require low amount of serum (1 mL) without tedious pre-treatment. The quantification of fenthion was carried out by gas chromatography-mass spectrometry and the recoveries ranged from 79 to 104% at two spiking levels for 6 replicates. Detection and quantification limits were calculated as 1.51 and 4.54 ng/mL of serum respectively. Two fenthion metabolites fenoxon and fenthion-sulfoxide were also identified.