Sequencing and comparative analysis of the Amblyomma sculptum mitogenome

• Published in: Veterinary parasitology Vol. 247; pp. 121 - 128
• Main Authors: de Lima, Paulo Henrique Costa, Barcelos, Rafael Mazioli, Klein, Raphael Contelli, Vidigal, Pedro Marcus Pereira, Montandon, Carlos Emmanuel, Fabres-Klein, Mary Hellen, Dergam, Jorge Abdala, Mafra, Cláudio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30-11-2017
• Subjects: Amblyomma cajennense complex; Amblyomma sculptum; Animals; Comparative genomics; Genome, Mitochondrial - genetics; Genomics; High-Throughput Nucleotide Sequencing; Ixodidae - genetics; Mitogenome; Molecular Sequence Annotation; Next generation sequencing; Sequence Analysis, DNA; Comparative genomics; Amblyomma sculptum; Amblyomma cajennense complex; Mitogenome; Next generation sequencing
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2017.10.007

•The first report of mitochondrial genome of Amblyomma sculptum.•A. sculptum and A. cajennense mitogenomes are same species with a few different SNPs.•ND5, control regions 1 and 2 genes are good molecular markers for lower taxon levels.
The mitogenome of Amblyomma sculptum was sequenced, providing important information for understanding the evolutionary relationships among species of the A. cajennense complex. The mitochondrial genome has a circular structure with 37 genes, including 13 coding DNA sequences, two ribosomal RNA genes (12S rRNA and 16S rRNA) and 22 tRNA genes. Comparative analysis with the mitogenomes of six reference species of the genus Amblyomma revealed that the ND5 gene, which is related to energy metabolism, and control regions 1 and 2 of the mitogenomes have polymorphisms that can be exploited as molecular markers to differentiate A. sculptum from other tick species in the Amblyomma cajennense complex as well as other Amblyomma species.