Misidentification of the SARS-CoV-2 Mu variant using commercial mutation screening assays

Detection of mutations by multiplex real-time RT-PCR is a widely used method for the screening of SARS-CoV-2 variants, but this method has several limitations. We describe three cases in which a Mu strain containing the mutation K417N was initially misclassified as the Beta variant. We recommend the...

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Bibliographic Details
Published in:	Archives of virology Vol. 167; no. 4; pp. 1141 - 1144
Main Authors:	Alejo-Cancho, Izaskun, Gual-de-Torrella, Ana, Gallego, Mikel, Urrutikoetxea-Gutierrez, Mikel, Lejarraga, Clara, López de Goikoetxea, Maria José
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01-04-2022 Springer Nature B.V
Subjects:	Biomedical and Life Sciences Biomedicine Brief Report COVID-19 - diagnosis Humans Infectious Diseases Medical Microbiology Multiplex Polymerase Chain Reaction Mutation Polymerase chain reaction SARS-CoV-2 - genetics Severe acute respiratory syndrome coronavirus 2 Virology
Online Access:	Get full text
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Summary:	Detection of mutations by multiplex real-time RT-PCR is a widely used method for the screening of SARS-CoV-2 variants, but this method has several limitations. We describe three cases in which a Mu strain containing the mutation K417N was initially misclassified as the Beta variant. We recommend the detection of P681H to distinguish between these two variants. Our experience highlights the importance of keeping track of new variants and mutations in order to adapt the current workflows.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Handling Editor: William G. Dundon.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-022-05395-w