Mechanisms and histopathological impacts of acetamiprid and azoxystrobin in male rats

• Published in: Environmental science and pollution research international Vol. 29; no. 28; pp. 43114 - 43125
• Main Authors: EL-Hak, Heba Nageh Gad, Al-Eisa, Rasha A., Ryad, Lamia, Halawa, Ekramy, El-Shenawy, Nahla S.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-06-2022; Springer Nature B.V
• Subjects: Animals; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Azoxystrobin; Catalase; Catalase - metabolism; Chemical pest control; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Fungicides; Glutathione; Glutathione reductase; Hormones; Insecticides; Male; Males; Mammals; Metabolism; Neonicotinoids - toxicity; Nitric oxide; Oxidative Stress; Parameters; Pest control; Pesticides; Pyrimidines; Rats; Reductases; Research Article; Rodents; Serum levels; Sex hormones; Sperm Motility; Spermatozoa; Strobilurins - pharmacology; Superoxide dismutase; Superoxide Dismutase - metabolism; Testes; Testis; Toxicity; Waste Water Technology; Water Management; Water Pollution Control; Lutilizing hormone; Follicle-stimulating hormone; Male rat; Calcium; Sperm count; Nitric oxide
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-021-18331-3

Acetamiprid (neonicotinoid insecticide) and azoxystrobin (fungicide) are widespread pesticides used for pest management, but they have the potential for toxicity to mammals. The goal of this study was to look for oxidative stress, metabolic alterations, and reproductive problems in male rats’ serum after 2 months of exposure to sub-lethal dosages of acetamiprid and azoxystrobin. Seven classes of male rats were formed: control, 3 groups of acetamiprid (1/10, 1/20, 1/40 LD
50
), and 3 groups of azoxystrobin (1/10, 1/20, 1/40 LD
50
) and were orally daily treated (
n
 = 8/group). Our findings revealed that acetamiprid and azoxystrobin disrupted oxidative and metabolic processes in the examined rats throughout 30 and 60 days of testing. The levels of nitric oxide increased significantly, while catalase, a superoxide dismutase enzyme, and glutathione reductase activity were reduced. Serum levels of sex hormones, calcium, and total protein have all dropped substantially in rats. In comparison to the control group, the testis and liver structure, as well as spermatozoa parameters, had distinct histological characteristics. In conclusion, acetamiprid and azoxystrobin exhibit dose- and time-dependent effects on oxidative parameters that cause testis damage.