Biochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazil

• Published in: Brazilian journal of microbiology Vol. 53; no. 1; pp. 349 - 358
• Main Authors: Aranha, Guilherme Mauro, Contato, Alex Graça, Salgado, José Carlos dos Santos, de Oliveira, Tássio Brito, Retamiro, Karina Miyuki, Ortolan, Guilherme Guimarães, Crevelin, Eduardo José, Nakamura, Celso Vataru, de Moraes, Luiz Alberto Beraldo, Peralta, Rosane Marina, Polizeli, Maria de Lourdes Teixeira de Moraes
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-03-2022; Springer Nature B.V
• Subjects: Additives; Agaricales; Amino acids; Animals; Antioxidants; Antioxidants - analysis; Antioxidants - pharmacology; Basidiocarps; Biological properties; Biomedical and Life Sciences; Brazil; Carbohydrates; Chlorocebus aethiops; Chromatography, Liquid; Cytotoxicity; Food additives; Food Microbiology; Functional foods & nutraceuticals; Fungal and Bacterial Physiology - Research Paper; Lepista sordida; Life Sciences; Macrophages; Medical Microbiology; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mushrooms; Mycelium - chemistry; Mycology; Phenolic compounds; Phenols; Plant Extracts - chemistry; Polyporaceae; Protocatechuic acid; Tandem Mass Spectrometry; Toxicity; Trametes - chemistry; Trametes hirsuta; Vero Cells; Brazil; Antioxidants; Cytotoxicity; Phenolic compounds; Mushrooms; Lepista sordida; Trametes hirsuta
• DOI: 10.1007/s42770-021-00670-5
• EISSN: 1678-4405
• ISSN: 1517-8382

The objective of this study was to evaluate the antioxidant activity, determine and quantify the phenolic compounds and other compounds, and evaluate the cellular cytotoxicity of mycelium extracts of two new Basidiomycete mushrooms strains isolated in Brazil and identified as
Lepista sordida
GMA-05 and
Trametes hirsuta
GMA-01. Higher amounts of proteins, free amino acids, total and reducing carbohydrates, and phenolic compounds as chlorogenic, ferulic, caffeic, and gallic acids were found in extracts of
T. hirsuta
and
L. sordida
. Protocatechuic acid was found only in aqueous extracts of
L. sordida.
The TLC of the extracts showed the predominance of glucose and smaller amounts of xylose. It was observed through UPLC-MS higher amounts of phenolic compounds. The aqueous extract from
T. hirsuta
had the most noteworthy results in the antioxidant assays, especially the ABTS test. The cytotoxic activity was evaluated using two different cell lineages and showed higher toxicity for
L. sordida
in macrophages J774-A1. However, in Vero cells, it was 12.6-fold less toxic when compared to
T. hirsuta.
Thus, both mushrooms show potential as functional foods or additives, presenting phenolic content, antioxidant activity, and low cytotoxic activity in the tested cells.
Graphical abstract