Rapid Purification of Human Bispecific Antibodies via Selective Modulation of Protein A Binding

Methods to rapidly generate high quality bispecific antibodies (BsAb) having normal half-lives are critical for therapeutic programs. Here, we identify 3 mutations (T307P, L309Q, and Q311R or “TLQ”) in the Fc region of human IgG1 which disrupt interaction with protein A while enhancing interaction w...

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Bibliographic Details
Published in:	Scientific reports Vol. 7; no. 1; pp. 15521 - 11
Main Authors:	Zwolak, Adam, Leettola, Catherine N., Tam, Susan H., Goulet, Dennis R., Derebe, Mehabaw G., Pardinas, Jose R., Zheng, Songmao, Decker, Rose, Emmell, Eva, Chiu, Mark L.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 14-11-2017 Nature Publishing Group
Subjects:	13/1 631/154/51 64/60 692/4017 82/1 82/80 82/83 Bispecific antibodies Humanities and Social Sciences Immunoglobulin G Monoclonal antibodies multidisciplinary Mutation pH effects Protein A Protein purification Proteins Science Science (multidisciplinary) Thermal stability
Online Access:	Get full text
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