Cardioprotective effects of insulin on carbon monoxide-induced toxicity in male rats

Carbon monoxide (CO) poisoning is a significant cause of death especially in developing countries. The current study investigated cardioprotective effects of insulin in CO-poisoned rats. Male rats were exposed to 3000 ppm CO for 1 h. Insulin (100 and 120 U/kg intraperitoneally) was immediately admin...

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Bibliographic Details
Published in:	Human & experimental toxicology Vol. 38; no. 1; p. 148
Main Authors:	Tabrizian, K, Shahriari, Z, Rezaee, R, Jahantigh, H, Bagheri, G, Tsarouhas, K, Docea, A O, Tsatsakis, A, Hashemzaei, M
Format:	Journal Article
Language:	English
Published:	England 01-01-2019
Subjects:	Animals Apoptosis - drug effects Carbon Monoxide Poisoning - drug therapy Carbon Monoxide Poisoning - metabolism Carbon Monoxide Poisoning - pathology Carbon Monoxide Poisoning - physiopathology Carboxyhemoglobin - analysis Cardiotonic Agents - therapeutic use Electrocardiography Insulin - therapeutic use Male Myocardium - metabolism Myocardium - pathology Proto-Oncogene Proteins c-bcl-2 - metabolism Rats, Wistar cardiotoxicity myocardial necrosis BCL2 carbon monoxide poisoning BAX apoptosis Akt Insulin
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Summary:	Carbon monoxide (CO) poisoning is a significant cause of death especially in developing countries. The current study investigated cardioprotective effects of insulin in CO-poisoned rats. Male rats were exposed to 3000 ppm CO for 1 h. Insulin (100 and 120 U/kg intraperitoneally) was immediately administered after CO exposure and on the next 4 days, on a daily basis (a total of 5 doses). On day 5, animals were euthanized, and the hearts were harvested for Western blotting and histopathological studies. The electrocardiograms (ECG) were recorded postexposure to CO and after the completion of insulin treatment period. Histopathological evaluations showed reduction of myocardial necrosis in insulin-treated animals compared to controls. BAX/BCL2 ratio, as a proapoptotic index, was significantly reduced in treatment groups ( p < 0.01). The ECG findings showed no differences among groups; also, compared to control animals, myocardial Akt levels were not markedly affected by insulin. The current study showed that insulin significantly reduces myocardial necrotic and apoptotic indices in CO-poisoned rats.
ISSN:	1477-0903
DOI:	10.1177/0960327118788134