Synergistic effect of Si-hydroxyapatite coating and VEGF adsorption on Ti6Al4V-ELI scaffolds for bone regeneration in an osteoporotic bone environment

• Published in: Acta biomaterialia Vol. 83; pp. 456 - 466
• Main Authors: Izquierdo-Barba, I., Santos-Ruiz, L., Becerra, J., Feito, M.J., Fernández-Villa, D., Serrano, M.C., Díaz-Güemes, I., Fernández-Tomé, B., Enciso, S., Sánchez-Margallo, F.M., Monopoli, D., Afonso, H., Portolés, M.T., Arcos, D., Vallet-Regí, M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-01-2019; Elsevier BV
• Subjects: Adsorption; Angiogenesis; Animals; Biocompatibility; Biomedical materials; Bone growth; Bone regeneration; Bone Regeneration - drug effects; Bones; Cell Line; Cell proliferation; Cell Proliferation - drug effects; Coating effects; Coatings; Durapatite - chemistry; Durapatite - pharmacology; Electron beam melting; Endothelial cells; Endothelial Cells - metabolism; Endothelial Cells - pathology; Female; Growth factors; Hydroxyapatite; Immersion coating; In vitro methods and tests; In vivo methods and tests; Mice; Osteoblasts; Osteoblasts - metabolism; Osteoblasts - pathology; Osteoporosis; Osteoporosis - drug therapy; Osteoporosis - metabolism; Osteoporosis - pathology; Regeneration; Regeneration (physiology); Scaffold; Scaffolds; Sheep; Silicon; Silicon - chemistry; Silicon - pharmacology; Silicon hydroxyapatite; Surgical implants; Swine; Synergistic effect; Titanium - chemistry; Titanium - pharmacology; Titanium base alloys; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - chemistry; Vascular Endothelial Growth Factor A - pharmacology; VEGF; Osteoporosis; Silicon hydroxyapatite; Scaffold; Bone regeneration; VEGF
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2018.11.017

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The osteogenic and angiogenic responses to metal macroporous scaffolds coated with silicon substituted hydroxyapatite (SiHA) and decorated with vascular endothelial growth factor (VEGF) have been evaluated in vitro and in vivo. Ti6Al4V-ELI scaffolds were prepared by electron beam melting and subsequently coated with Ca10(PO4)5.6(SiO4)0.4(OH)1.6 following a dip coating method. In vitro studies demonstrated that SiHA stimulates the proliferation of MC3T3-E1 pre-osteoblastic cells, whereas the adsorption of VEGF stimulates the proliferation of EC2 mature endothelial cells. In vivo studies were carried out in an osteoporotic sheep model, evidencing that only the simultaneous presence of both components led to a significant increase of new tissue formation in osteoporotic bone.
Reconstruction of bones after severe trauma or tumors extirpation is one of the most challenging tasks in the field of orthopedic surgery. This scenario is even more complicated in the case of osteoporotic patients, since their bone regeneration capability is decreased. In this work we present a porous implant that promotes bone regeneration even in osteoporotic bone. By coating the implant with osteogenic bioceramics such as silicon substituted hydroxyapatite and subsequent adsorption of vascular endothelial growth factor, these implants stimulate the bone ingrowth when they are implanted in osteoporotic sheep.