Candida albicans and Candida glabrata triosephosphate isomerase – a moonlighting protein that can be exposed on the candidal cell surface and bind to human extracellular matrix proteins

• Published in: BMC microbiology Vol. 21; no. 1; pp. 1 - 199
• Main Authors: Satala, Dorota, Satala, Grzegorz, Zawrotniak, Marcin, Kozik, Andrzej
• Format: Journal Article
• Language: English
• Published: London BioMed Central 01-07-2021; BMC
• Subjects: Antibodies; Candida; Cell surface; Cell walls; Colonization; Crosslinking; Enzymes; Extracellular matrix; fibronectin; Fungi; Glycolysis; Growth conditions; Hypotheses; Infections; Metabolism; Microorganisms; moonlighting proteins; non-albicans Candida species; Peptides; Proteins; Structural models; Surface plasmon resonance; Synthetic peptides; Triose-phosphate isomerase; triosephosphate isomerase; Vitronectin; Yeast; Yeasts
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-021-02235-w

Background Triosephosphate isomerase (Tpi1) is a glycolytic enzyme that has recently been reported also to be an atypical proteinaceous component of the Candida yeast cell wall. Similar to other known candidal “moonlighting proteins”, surface-exposed Tpi1 is likely to contribute to fungal adhesion during the colonization and infection of a human host. The aim of our present study was to directly prove the presence of Tpi1 on C. albicans and C. glabrata cells under various growth conditions and characterize the interactions of native Tpi1, isolated and purified from the candidal cell wall, with human extracellular matrix proteins. Results Surface plasmon resonance measurements were used to determine the dissociation constants for the complexes of Tpi1 with host proteins and these values were found to fall within a relatively narrow range of 10− 8-10− 7 M. Using a chemical cross-linking method, two motifs of the Tpi1 molecule (aa 4–17 and aa 224–247) were identified to be directly involved in the interaction with vitronectin. A proposed structural model for Tpi1 confirmed that these interaction sites were at a considerable distance from the catalytic active site. Synthetic peptides with these sequences significantly inhibited Tpi1 binding to several extracellular matrix proteins suggesting that a common region on the surface of Tpi1 molecule is involved in the interactions with the host proteins. Conclusions The current study provided structural insights into the interactions of human extracellular matrix proteins with Tpi1 that can occur at the cell surface of Candida yeasts and contribute to the host infection by these fungal pathogens.