Multiplexed detection of viral infections using rapid in situ RNA analysis on a chip

Viral infections are a major cause of human disease, but many require molecular assays for conclusive diagnosis. Current assays typically rely on RT-PCR or ELISA; however, these tests often have limited speed, sensitivity or specificity. Here, we demonstrate that rapid RNA FISH is a viable alternati...

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Bibliographic Details
Published in:	Lab on a chip Vol. 15; no. 15; p. 3170
Main Authors:	Shaffer, Sydney M, Joshi, Rohan P, Chambers, Benjamin S, Sterken, David, Biaesch, Andrew G, Gabrieli, David J, Li, Yang, Feemster, Kristen A, Hensley, Scott E, Issadore, David, Raj, Arjun
Format:	Journal Article
Language:	English
Published:	England 07-08-2015
Subjects:	Algorithms Animals Dogs HeLa Cells Humans Image Processing, Computer-Assisted - methods In Situ Hybridization, Fluorescence - methods Madin Darby Canine Kidney Cells Microfluidic Analytical Techniques - instrumentation Point-of-Care Systems RNA Virus Infections - diagnosis RNA Virus Infections - virology RNA Viruses - genetics RNA Viruses - isolation & purification RNA, Viral - analysis RNA, Viral - genetics ROC Curve
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