Oxidative stress induced in Hyalella azteca by an effluent from a NSAID-manufacturing plant in Mexico

• Published in: Ecotoxicology (London) Vol. 25; no. 7; pp. 1288 - 1304
• Main Authors: Novoa-Luna, Karen Adriana, Romero-Romero, Rubí, Natividad-Rangel, Reyna, Galar-Martínez, Marcela, SanJuan-Reyes, Nely, García-Medina, Sandra, Martínez-Vieyra, Catalina, Neri-Cruz, Nadia, Gómez-Oliván, Leobardo Manuel
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-09-2016; Springer; Springer Nature B.V
• Subjects: Amphipoda - physiology; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents, Non-Steroidal - toxicity; Biomarkers - metabolism; Carbonyl compounds; Catalase - metabolism; Contaminants; Detergents; Disinfectants; Earth and Environmental Science; Ecology; Ecotoxicology; Environment; Environmental Management; Environmental Monitoring; Enzymes; Glutathione Peroxidase - metabolism; Hyalella azteca; Industrial effluents; Industrial wastewater; Lipid Peroxidation - drug effects; Manufacturing and Industrial Facilities; Manufacturing industry; Mexico; Nonsteroidal anti-inflammatory drugs; Oxidative Stress; Peroxidation; Pharmaceutical industry; Protein Carbonylation - drug effects; Sodium hypochlorite; Superoxide; Superoxide Dismutase - metabolism; Waste Water - chemistry; Wastewater; Water Pollutants, Chemical - toxicity; Mexico; Oxidative stress; Nonsteroidal anti-inflammatory drugs; NaClO; Amphipod; Hyalella azteca
• ISSN: 0963-9292; 1573-3017
• DOI: 10.1007/s10646-016-1682-2

Production in the pharmaceutical industry has increased and along with it, the amount of wastewater of various characteristics and contaminant concentrations. The main chemicals in these effluents are solvents, detergents, disinfectants—such as sodium hypochlorite (NaClO)—and pharmaceutical products, all of which are potentially ecotoxic. Therefore, this study aimed to evaluate the oxidative stress induced in the amphipod
Hyalella azteca
by the effluent from a nonsteroidal anti-inflammatory drug (NSAID)-manufacturing plant. The median lethal concentration (72 h-LC
50
) was determined and
H. azteca
were exposed to the lowest observed adverse effect level (0.0732 %) for 12, 24, 48 and 72 h, and biomarkers of oxidative stress were evaluated [hydroperoxide content (HPC), lipid peroxidation (LPX), protein carbonyl content (PCC), and the activity of the superoxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)]. Statistically significant increases with respect to the control group (
P
 < 0.05) were observed in HPC, LPX and PCC in
H. azteca
at all exposure times. Antioxidant enzymes activity SOD, CAT and GPx activity also increased significantly (
P
 < 0.05) with respect to the control group. In conclusion, the industrial effluent analyzed in the present study contains NSAIDs and NaClO, and induces oxidative stress in
H. azteca.