On-line control of an immobilized hybridoma culture with multi-channel flow injection analysis

An immobilized hybridoma cell line was cultivated at controlled glucose and glutamine concentrations. On-line analysis of the substrates was carried out with a multi-channel flow injection analysis system. The analysis system also determined on-line the lactate and ammonium concentration. The substr...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of biotechnology Vol. 43; no. 3; pp. 229 - 242
Main Authors:	van der Pol, Jens J., Joksch, Burkhard, Galgens, Jochem, Biselli, Manfred, de Gooijer, Cornells D., Tramper, Johannes, Wandrey, Christian
Format:	Journal Article
Language:	English
Published:	Lausanne Elsevier B.V 15-12-1995 Amsterdam Elsevier New York, NY
Subjects:	Animal cells Animals Antibodies, Monoclonal - biosynthesis Biological and medical sciences Biotechnology Biotechnology - instrumentation Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures FIA Fluidized-bed reactor, Glucose Fundamental and applied biological sciences. Psychology Glucose - administration & dosage Glucose - metabolism Glutamine Glutamine - administration & dosage Glutamine - metabolism Hybridoma Hybridomas - cytology Hybridomas - immunology Hybridomas - metabolism Kinetics Lactates - metabolism Lactic Acid Methods. Procedures. Technologies Models, Biological On-line control Quaternary Ammonium Compounds - metabolism Sectie Proceskunde Sub-department of Food and Bioprocess Engineering Hybridoma On-line control Fluidized-bed reactor, Glucose FIA Glutamine Fluidized bed reactor Cell culture On line Control system Immobilized cell Glucose Adaptive control Bioreactor Cell line Animal Flow injection
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!