Anti-Pigmentary Effect of (-)-4-Hydroxysattabacin from the Marine-Derived Bacterium Bacillus sp

• Published in: Marine drugs Vol. 15; no. 5; p. 138
• Main Authors: Kim, Kyuri, Leutou, Alain S, Jeong, Haein, Kim, Dayoung, Seong, Chi Nam, Nam, Sang-Jip, Lim, Kyung-Min
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13-05-2017; MDPI
• Subjects: Activation; Animals; Aquatic Organisms; Bacillus - metabolism; Bacteria; Biochemistry; Biocompatibility; Biomedical materials; Cell culture; Cell Line, Tumor; Cell Survival; Cells; DNA; Dopachrome isomerase; Dose-Response Relationship, Drug; Enzymatic activity; Epidermis - drug effects; Genes; Hexanones - chemistry; Hexanones - metabolism; Hexanones - pharmacology; Hormones; Humans; Levodopa - administration & dosage; Levodopa - pharmacology; Melanin; Melanins - metabolism; Melanoma; Melanoma - metabolism; Mice; Molecular Structure; Nucleotide sequence; PCR; Pigmentation - drug effects; Polymerase chain reaction; Proteins; Real time; Regression; Skin; Surgical implants; Three dimensional models; Tissue; Transcription; Tyrosinase; Tyrosinase-related protein 1; melanogenesis; (-)-4-hydroxysattabacin; marine-derived; Bacillus sp; anti-pigmentary effect
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md15050138

Bioactivity-guided isolation of a crude extract from a culture broth of Bacillus sp. has led to the isolation of (-)-4-hydroxysattabacin (1). The inhibitory effect of (-)-4-hydroxysattabacin (1) was investigated on melanogenesis in the murine melanoma cell line, B16F10, and human melanoma cell line, MNT-1, as well as a pigmented 3D-human skin model. (-)-4-Hydroxysattabacin treatment decreased melanin contents in a dose-dependent manner in α-melanocyte stimulating hormone (α-MSH)-stimulated B16F10 cells. Quantitative real time PCR (qRT-PCR) demonstrated that treatment with (-)-4-hydroxysattabacin down-regulated several melanogenic genes, including tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) while their enzymatic activities were unaffected. The anti-melanogenic effects of (-)-4-hydroxysattabacin were further demonstrated in a pigmented 3D human epidermal skin model, MelanodermTM, and manifested as whitening and regression of melanocyte activation in the tissue.