Ovarian steroids differentially regulate the expression of PRL-R in neuroendocrine dopaminergic neuron populations: a double label confocal microscopic study

• Published in: Brain research Vol. 802; no. 1; pp. 141 - 154
• Main Authors: Lerant, Anna, Freeman, Marc E
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 17-08-1998; Amsterdam Elsevier; New York, NY
• Subjects: Animals; Arcuate nucleus; Arcuate Nucleus of Hypothalamus - cytology; Arcuate Nucleus of Hypothalamus - metabolism; Biological and medical sciences; Dopamine - physiology; Dopaminergic neuron; Estradiol; Estradiol - blood; Estradiol - physiology; Female; Fundamental and applied biological sciences. Psychology; General aspects. Hormone interactions. Hormone actions on several organ systems. Adaptive reactions; Hypothalamus; Hypothalamus - cytology; Hypothalamus - metabolism; Hypothalamus, Middle - cytology; Hypothalamus, Middle - metabolism; Intracellular Membranes - metabolism; Microscopy, Confocal; Neuroendocrine regulation; Neurons - metabolism; Neurosecretory Systems - cytology; Neurosecretory Systems - metabolism; Ovariectomy; Periventricular nucleus; Progesterone; Progesterone - blood; Progesterone - physiology; Prolactin - blood; Prolactin - physiology; Prolactin receptor; Rats; Rats, Sprague-Dawley; Receptors, Prolactin - metabolism; Tissue Distribution; Vertebrates: endocrinology; Arcuate nucleus; Neuroendocrine regulation; Dopaminergic neuron; Periventricular nucleus; Hypothalamus; Progesterone; Prolactin receptor; Estradiol; Rat; Rodentia; Central nervous system; Ovarian hormone; Vertebrata; Mammalia; Adenohypophyseal hormone; Prolactin; Sex steroid hormone; Brain (vertebrata); Hormonal receptor
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(98)00583-6

The aims of this study were (1) to identify the possible
hypothalamic targets for a short prolactin (PRL) feedback in the adult female rat by identifying DAergic neuron populations expressing PRL receptor (PRL-R); (2) to describe the
effect of ovarian steroids on the expression of PRL-R and (3) to compare the
distribution of both the extracellular (EC) and ligand binding (LB) domains of the PRL-R on the hypothalamic dopaminergic neurons by applying double label immunocytochemistry for the different domains of PRL-R and for tyrosine hydroxylase (TH). Five- to six-month-old female rats were ovariectomized (OVX) and implanted with either 17β-estradiol (E
2), progesterone (P
4) or received an E
2 and a P
4 implant (E
2+P
4) at the same time. In the periventricular nucleus and in the dorsomedial portion of the middle arcuate nucleus, a dramatic increase in PRL-R
EC immunoreactivity was observed in E
2 implanted rats. This increase was attenuated in E
2+P
4 rats, but P
4 treatment alone had no effect. Changes in PRL-R
EC expression were paralleled by changes in serum PRL levels. Interestingly, PRL-R
EC expression in the rostral arcuate nucleus decreased in P
4 implanted rats, however, P
4 did not attenuate the E
2-induced increase in PRL-R
EC density. PRL-R
EC immunostaining was observed on the membrane, in the cytoplasm and in the nucleus. PRL-R
LB immunoreactivity was also detectable in the TH positive neurons, but no nuclear staining was observed with this antibody. However, we found a strong PRL-R
LB immunostaining in the ependymal lining of the 3rd ventricle and in the processes of tanycytes projecting to the median eminence. These data indicate that (1) all neuroendocrine DAergic cells can be targets for PRL, (2) expression of PRL-R is differentially affected by ovarian steroids in the different TH cell populations, (3) PRL-R
LB domain may be involved in trafficking PRL in the median eminence.