The Effect of Phloroglucinol, A Component of Ecklonia cava Extract, on Hepatic Glucose Production

• Published in: Marine drugs Vol. 15; no. 4; p. 106
• Main Authors: Yoon, Ji-Young, Choi, Hojung, Jun, Hee-Sook
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05-04-2017; MDPI
• Subjects: Algae; AMP; AMP-activated protein kinase; AMP-Activated Protein Kinases - metabolism; AMPKα; Animals; Blood; Blood Glucose - drug effects; Cells; Diabetes mellitus; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - drug therapy; Diabetes Mellitus, Type 2 - metabolism; Diet; Diet, High-Fat; Enzymes; G6Pase; Gene expression; Gluconeogenesis; Gluconeogenesis - drug effects; Glucose; Glucose - metabolism; Glucose tolerance; Glucose-6-phosphatase; Glucose-6-Phosphatase - metabolism; Hep G2 Cells; Hepatocytes; Hepatocytes - drug effects; Hepatocytes - metabolism; High fat diet; Humans; Hypoglycemic Agents - pharmacology; Kinases; Liver; Liver - drug effects; Liver - metabolism; Male; Mice; Mice, Inbred C57BL; mRNA; PEPCK; Phaeophyceae - chemistry; Pharmacology; Phenolic compounds; Phenols; Phloroglucinol; Phloroglucinol - pharmacology; Phosphatase; Phosphoenolpyruvate Carboxykinase (ATP) - metabolism; Phosphorylation - drug effects; Proteins; Pyruvic acid; Signal transduction; Signal Transduction - drug effects; Tissue; AMPKα; G6Pase; PEPCK; gluconeogenesis; Phloroglucinol
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md15040106

Phloroglucinol is a phenolic compound that is one of the major compounds in
(brown alga). It has many pharmacological activities, but its anti-diabetic effect is not yet fully explored. In this study, we investigated the effect of phloroglucinol on the control of blood glucose levels and the regulation of hepatic glucose production. Phloroglucinol significantly improved glucose tolerance in male C57BL/6J mice fed a high fat diet (HFD) and inhibited glucose production in mouse primary hepatocytes. The expression of phosphoenol pyruvate carboxykinase (PEPCK) and glucose-6-phosphatase mRNA and protein (G6Pase), enzymes involved in gluconeogenesis, were inhibited in liver tissue from phloroglucinol-treated mice and in phloroglucinol-treated HepG2 cells. In addition, phloroglucinol treatment increased phosphorylated AMP-activated protein kinase (AMPK)α in HepG2 cells. Treatment with compound C, an AMPKα inhibitor, inhibited the increase of phosphorylated AMPKα and the decrease of PEPCK and G6Pase expression caused by phloroglucinol treatment. We conclude that phloroglucinol may inhibit hepatic gluconeogenesis via modulating the AMPKα signaling pathway, and thus lower blood glucose levels.