Daily consumption of black soybean (Glycine max L.) seed coat polyphenols attenuates dyslipidemia in apolipoprotein E-deficient mice

• Published in: Journal of functional foods Vol. 72; p. 104054
• Main Authors: Tanaka, Wataru, Matsuyama, Hiroki, Yokoyama, Daigo, Yamashita, Yoko, Ashida, Hitoshi, Sakono, Masanobu, Sakakibara, Hiroyuki
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-09-2020; Elsevier
• Subjects: apolipoprotein E-deficient mice; Black soybean (Glycine max L.) seed coat; Cholesterol; Cyanidin-3-glucoside; Dyslipidaemia; Triglyceride; apolipoprotein E-deficient mice; BE; Cyanidin-3-glucoside; EGCG; Black soybean (Glycine max L.) seed coat; CN; Triglyceride; Cholesterol; GIP; Fasn; Dyslipidaemia; ApoE; Pparα; Cyp7a1
• ISSN: 1756-4646; 2214-9414
• DOI: 10.1016/j.jff.2020.104054

Daily consumption of black soybean seed coat polyphenols attenuates dyslipidemia in apolipoprotein E-deficient mice.
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•Black soybean seed coat extract (BE) rich in polyphenols, such as cyanidin glucoside.•Apolipoprotein E-deficient (ApoE−/−) mice spontaneously develop dyslipidemia.•BE lowered serum and hepatic total cholesterol and triglyceride levels in ApoE−/− mice.•These were characteristic in ApoE−/− mice, but not in their corresponding wild-type.
Black soybean (Glycine max L.) seed coat extract (BE) is rich in polyphenols, including epicatechin, procyanidin, and cyanidin-3-glucoside. We evaluated the effects of daily consumption of BE in apolipoprotein E-deficient (ApoE−/−) mice. ApoE−/− and wild-type BALB/c mice were fed a 20% fat diet with or without 1% BE for 8 weeks. Bodyweight was unaffected by BE. BE supplementation significantly lowered serum and hepatic total cholesterol and hepatic triglyceride levels. Hepatic mRNA expression of cholesterol 7α-hydroxylase (Cyp7a1) and peroxisome proliferator-activated receptor α (Pparα) were significantly stimulated in ApoE−/− mice consuming the BE diet. These effects were characteristic in ApoE−/− mice, but not in their corresponding wild type. In conclusion, it is clear that dietary consumption of BE affected blood and hepatic levels of cholesterol and triglyceride in ApoE−/− mice, and consequently exerted preventive effects for dyslipidemia. Putative mechanisms include the upregulation of hepatic Cyp7a1 and Pparα mRNA expression.