Ultra-Performance Liquid Chromatography-Ion Mobility Separation-Quadruple Time-of-Flight MS (UHPLC-IMS-QTOF MS) Metabolomics for Short-Term Biomarker Discovery of Orange Intake: A Randomized, Controlled Crossover Study

• Published in: Nutrients Vol. 12; no. 7; p. 1916
• Main Authors: Lacalle-Bergeron, Leticia, Portolés, Tania, López, Francisco J, Sancho, Juan Vicente, Ortega-Azorín, Carolina, Asensio, Eva M, Coltell, Oscar, Corella, Dolores
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29-06-2020; MDPI
• Subjects: Adult; Bias; Biological properties; Biological samples; Biomarkers; Biomarkers - blood; Chromatography; Chromatography, High Pressure Liquid - methods; Citrus; Citrus fruits; Cross-Over Studies; Diet Surveys - methods; Dietary intake; Eating - physiology; Epidemiology; Female; Flavonoids; Food; Fruits; Healthy Volunteers; Humans; Intervention; ion mobility; Ion Mobility Spectrometry - methods; Ionic mobility; Liquid chromatography; Male; Mass spectrometry; Mass Spectrometry - methods; Mass spectroscopy; Metabolism; Metabolites; Metabolomics; Metabolomics - methods; Mobility; New technology; NMR; Nuclear magnetic resonance; Nutrition; orange intake; Oranges; Plasma; Questionnaires; Separation; Urine; United States > US; Spain; ion mobility; metabolomics; biomarkers; orange intake
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu12071916

A major problem with dietary assessments is their subjective nature. Untargeted metabolomics and new technologies can shed light on this issue and provide a more complete picture of dietary intake by measuring the profile of metabolites in biological samples. Oranges are one of the most consumed fruits in the world, and therefore one of the most studied for their properties. The aim of this work was the application of untargeted metabolomics approach with the novel combination of ion mobility separation coupled to high resolution mass spectrometry (IMS-HRMS) and study the advantages that this technique can bring to the area of dietary biomarker discovery, with the specific case of biomarkers associated with orange consumption (
) in plasma samples taken during an acute intervention study (consisting of a randomized, controlled crossover trial in healthy individuals). A total of six markers of acute orange consumption, including betonicines and conjugated flavonoids, were identified with the experimental data and previous literature, demonstrating the advantages of ion mobility in the identification of dietary biomarkers and the benefits that an additional structural descriptor, as the collision cross section value (CCS), can provide in this area.