WNT-associated gene expression in human mesenchymal stromal cells under hypoxic stress

• Published in: Doklady. Biochemistry and biophysics Vol. 465; no. 1; pp. 354 - 357
• Main Authors: Udartseva, O. O., Andreeva, E. R., Buravkova, L. B.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-11-2015; Springer Nature B.V
• Subjects: Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biophysics and Molecular Biology; Cell Hypoxia; Cells, Cultured; Cellular biology; Gene expression; Gene Expression Profiling; Humans; Hypoxia; Life Sciences; Mesenchymal Stromal Cells - metabolism; Oxygen - metabolism; Signal transduction; Stress, Physiological; Wnt Signaling Pathway; DOKLADY Biochemistry; Planar Cell Polarity; SFRP4 Gene; Mesenchymal Stromal Cell; Hypoxic Stress
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672915060046

The effect of short-term stress on the expression of 84 Wnt-related genes in mesenchymal stromal cells (MSCs) was analyzed. In MSCs permanently expanded at 5% O
2
,
Dkk1
,
RHOA
,
CSNK1A1
,
CSNK2A1
,
GSK3
β, and
PORCN
genes were most highly expressed.
FZD1
,
FZD3
,
SFRP1
, and
SFRP4
genes were up-regulated after short-term hypoxic stress (0.1% O
2
), whereas the expression of
LEF-1
and
RUVBL1
genes was significantly inhibited (down-regulated). The data suggest that Wnt signal transduction was suppressed under hypoxic stress, whereas the noncanonical Wnt signaling prevailed under the “physiological hypoxia” (5% O
2
).