Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments

• Published in: International biodeterioration & biodegradation Vol. 84; pp. 161 - 167
• Main Authors: Pedetta, Andrea, Pouyte, Karina, Herrera Seitz, María K., Babay, Paola A., Espinosa, Mariela, Costagliola, Marcela, Studdert, Claudia A., Peressutti, Silvia R.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-10-2013
• Subjects: Bioavailability; Bioremediation; Biosurfactant; Carbon; Chemotaxis; Degradation; energy; microorganisms; Phenanthrene; Polycyclic aromatic hydrocarbon; polycyclic aromatic hydrocarbons; Pseudomonas; ribosomal RNA; Searching; Sediments; sodium salicylate; Sphingomonas; Strain; Strategy; surfactants; Argentina; Biosurfactant; Phenanthrene; Bioremediation; Chemotaxis; Polycyclic aromatic hydrocarbon
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2012.04.018

Samborombón Bay, Argentina, is a highly productive area exposed to chronic contamination, including polycyclic aromatic hydrocarbons. Four phenanthrene-degrading strains were isolated from sediments collected in this area. Analysis of partial 16S rRNA sequencing and a BLAST search indicated that three of the strains belong to genus Pseudomonas and one to Sphingomonas. All the strains were able to grow in 150 mg l−1 phenanthrene as the sole carbon and energy source, with high degradation efficiency (75–100% in 72–168 h). Growth in sodium salicylate indicated that the Pseudomonas strains used this pathway to degrade phenanthrene.
Strategies that may enhance substrate bioavailability, such as surfactant production and chemotactic responses, were tested. Two Pseudomonas strains showed significant production of surface-active compounds, and a strong chemotactic response toward phenanthrene. Together with the ability to consume the supplied phenanthrene to completion, these characteristics make the mentioned strains good candidates for bioremediation strategies intended to clean up polluted areas.
► Four phenanthrene-degrading strains were isolated from Samborombón Bay (Argentina). ► Three of the strains belong to genus Pseudomonas sp. and one to Sphingomonas sp. ► The isolated strains degraded phenanthrene with high efficiency. ► Some strains produced biosurfactants and showed chemotaxis toward phenanthrene.