Probing prenatal bisphenol exposures and tissue-specific DNA methylation responses in cord blood, cord tissue, and placenta

• Published in: Reproductive toxicology (Elmsford, N.Y.) Vol. 115; pp. 74 - 84
• Main Authors: McCabe, Carolyn F., Goodrich, Jaclyn M., Bakulski, Kelly M., Domino, Steven E., Jones, Tamara R., Colacino, Justin, Dolinoy, Dana C., Padmanabhan, Vasantha
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-2023
• Subjects: Cord Blood; Cord Tissue; DNA Methylation; Environmental epigenomics; Female; Fetal Blood; Humans; Infant; Pilot Projects; Placenta; Pregnancy; Prenatal exposure; Prenatal Exposure Delayed Effects - genetics; Environmental epigenomics; MMIP; BPS; Prenatal exposure; CT; Cord Blood; BPA; Placenta; DNA methylation; BPF; Cord Tissue; PL; CB
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2022.11.005

The early-gestational fetal epigenome establishes the landscape for fetal development and is susceptible to disruption via environmental stressors including chemical exposures. Research has explored how cell- and tissue-type-specific epigenomic signatures contribute to human disease, but how the epigenome in each tissue comparatively responds to environmental exposures is largely unknown. This pilot study compared DNA methylation in four previously identified genes across matched cord blood (CB), cord tissue (CT), and placental (PL) samples from 28 mother-infant pairs in tthe Michigan Mother Infant Pairs study; evaluated association between prenatal exposure to bisphenols (BPA, BPF, and BPS) and DNA methylation (DNAm) by tissue type; compared epigenome-wide DNAm of CB and PL; and explored associations between prenatal bisphenol exposures and epigenome-wide DNAm in PL. Bisphenol concentrations were quantified in first-trimester maternal urine. DNAm was assessed at four genes via pyrosequencing in three tissues; epigenome-wide DNAm analysis via Infinium MethylationEPIC array was completed on CB and PL. Candidate gene analysis revealed tissue-specific differences across all genes. In adjusted linear regression, BPA and BPF were associated with DNAm across candidate genes in PL but not CB and CT. Epigenome-wide comparison of matched CB and PL DNAm revealed tissue-specific differences at most CpG sites and modest associations between maternal first-trimester bisphenol exposures and PL but not CB DNAm. These data endorse inclusion of a variety of tissues in prenatal exposure studies. Overlapping and divergent responses in CB, CT, and PL demonstrate their utility in combination to capture a fuller picture of the epigenetic effect of developmental exposures.
•Candidate gene analysis revealed tissue-specific differences across all four genes.•600,000 + differentially methylated sites were detected between cord blood and placenta.•Epigenome-wide placenta DNAm was associated with maternal 1st-trimester bisphenols.