Calsenilin is required for endocrine pancreas development in zebrafish

• Published in: Developmental dynamics Vol. 236; no. 6; pp. 1517 - 1525
• Main Authors: Stetsyuk, V., Peers, B., Mavropoulos, A., Verbruggen, V., Thisse, B., Thisse, C., Motte, P., Duvillié, B., Scharfmann, R.
• Format: Journal Article Web Resource
• Language: English
• Published: New York Wiley‐Liss, Inc 01-06-2007; Wiley; Wiley Liss, Inc
• Subjects: Animals; Animals, Genetically Modified; Biochemistry, biophysics & molecular biology; Biochemistry, Molecular Biology; Biochimie, biophysique & biologie moléculaire; calsenilin; Danio rerio; development; Embryo, Nonmammalian - embryology; Embryo, Nonmammalian - metabolism; Embryo, Nonmammalian/embryology/metabolism; Endocrine System - embryology; Endocrine System - metabolism; Endocrine System/embryology/metabolism; Gene Expression Regulation, Developmental; Kv Channel-Interacting Proteins - genetics; Kv Channel-Interacting Proteins - metabolism; Kv Channel-Interacting Proteins/genetics/metabolism; Life Sciences; Molecular biology; Mutation - genetics; pancreas; Pancreas - embryology; Pancreas - metabolism; Pancreas/embryology/metabolism; Pancreatic Hormones - metabolism; Receptors, Notch - metabolism; Sciences du vivant; Signal Transduction; Tretinoin - metabolism; zebrafish; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - metabolism; Zebrafish/embryology/genetics/metabolism
• ISSN: 1058-8388; 1097-0177; 1097-0177
• DOI: 10.1002/dvdy.21149

Calsenilin/DREAM/Kchip3 is a neuronal calcium‐binding protein. It is a multifunctional protein, mainly expressed in neural tissues and implicated in regulation of presenilin processing, repression of transcription, and modulation of A‐type potassium channels. Here, we performed a search for new genes expressed during pancreatic development and have studied the spatiotemporal expression pattern and possible role of calsenilin in pancreatic development in zebrafish. We detected calsenilin transcripts in the pancreas from 21 somites to 39 hours postfertilization stages. Using double in situ hybridization, we found that the calsenilin gene was expressed in pancreatic endocrine cells. Loss‐of‐function experiments with anti‐calsenilin morpholinos demonstrated that injected morphants have a significant decrease in the number of pancreatic endocrine cells. Furthermore, the knockdown of calsenilin leads to perturbation in islet morphogenesis, suggesting that calsenilin is required for early islet cell migration. Taken together, our results show that zebrafish calsenilin is involved in endocrine cell differentiation and morphogenesis within the pancreas. Developmental Dynamics 236:1517–1525, 2007. © 2007 Wiley‐Liss, Inc.