Remote ischemic conditioning protects against testicular ischemia∕reperfusion injury in rats

• Published in: Acta cirurgica brasileira Vol. 35; no. 2; p. e202000203
• Main Authors: Damasceno, Ananda Vitória Barros Suzuki, Barros, Charles Alberto Villacorta de, Percario, Sandro, Ribeiro Junior, Rubens Fernando Gonçalves, Monteiro, Andrew Moraes, Gouveia, Eduardo Henrique Herbster, Henriques, Higor Yuri Bezerra
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01-01-2020
• Subjects: Acetylcysteine; Acetylcysteine - pharmacology; Animals; Drug Evaluation, Preclinical; Free Radical Scavengers - pharmacology; Ischemia; Ischemic Preconditioning - methods; Male; Oxidative Stress - drug effects; Oxygen Radical Absorbance Capacity; Random Allocation; Rats; Rats, Wistar; Reperfusion; Reperfusion Injury; Spermatic Cord Torsion; Testis; Testis - blood supply; Testis - drug effects
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/s0102-865020200020000003

Purpose To evaluate the effect of remote ischemic conditioning associated to N-acetylcysteine (NAC) on testicular ischemia∕reperfusion (I∕R) injury in rats. Methods Twenty-five adult male Wistar rats were randomly distributed into five experimental groups (n=5), as follows: Sham, I∕R, Perconditioning (PER), NAC and PER+NAC. Two-hour ischemia was induced by rotating the left testis 720° to clockwise direction, followed by 4 hours of reperfusion. Perconditioning was performed by three I/R cycles of 10 min each on the left limb, 30 min before reperfusion. N-acetylcysteine (150 mg∕kg) was administered 30 min before reperfusion. Results Statistical differences were observed in MDA levels between I/R group with all groups (p<0.01), in addition there was statistical difference between PER and Sham, and PER+ NAC groups (p<0.05) in plasma. Conclusions The protective effect of perconditioning isolated in the reduction of lipid peroxidation related to oxidative stress was demonstrated. However, when Perconditioning was associated with NAC, there was no protective effect against testicular injury of ischemia and reperfusion.