Morphological and immunohistochemical analysis of proteins CASPASE 3 and XIAP in rats subjected to cerebral ischemia and chronic alcoholism

• Published in: Acta cirurgica brasileira Vol. 33; no. 8; pp. 652 - 663
• Main Authors: Schiavoni, Vagner Sarraipo, Silva, Jairo Pinheiro da, Lizarte Neto, Fermino Sanches, Assis, Múcio Luiz Cirino de, Tazima, Maria de Fátima Galli Sorita, Carvalho, Camila Albuquerque Melo de, Tirapelli, Daniela Pretti da Cunha, Carlotti, Jr, Carlos Gilberto, Colli, Benedicto Oscar, Tirapelli, Luis Fernando
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 01-08-2018
• Subjects: Alcoholism; Alcoholism - metabolism; Alcoholism - pathology; Animals; Apoptosis; Brain Ischemia; Caspase 3 - analysis; Edema; Electromyography - methods; Immunohistochemistry; Inhibitor of Apoptosis Proteins - analysis; Ischemic Attack, Transient - metabolism; Ischemic Attack, Transient - pathology; Male; Microscopy, Electron, Transmission; Middle Cerebral Artery; Mitochondria - pathology; Morphology; Random Allocation; Rats; Rats, Wistar; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Time Factors
• ISSN: 0102-8650; 1678-2674
• DOI: 10.1590/s0102-865020180080000001

To evaluate histopathological and ultrastructural changes and expression of proteins related to apoptosis CASPASE 3 and XIAP after experimental induction of temporary focal cerebral ischemia (90 minutes) due to obstruction of the middle cerebral artery in alcoholism model.
Forty adult Wistar rats were used, subdivided into 5 experimental groups: control group (C); Sham group (S); Ischemic group (I); Alcoholic group (A); and Ischemic and Alcoholized group (I+A): animals submitted to the same treatment of group A and after four weeks were submitted to focal cerebral ischemia during 90 minutes, followed by reperfusion of 48 hours. Were processed for histopathological analysis and immunohistochemistry (for the protein expression of CASPASE -3 and XIAP).
Greater histopathological changes were observed in the animals of groups I and I+A in the three areas analyzed. The neuronal loss was higher in the medial striatum region of the animals of groups I and I + A. The protein expression of CASPASE -3 was higher than that of XIAP in the groups I and I + A for both proteins.
The expression of XIAP was slightly higher where the histopathological changes and expression of CASPASE -3 was less evident.