Differentiation of Mesenchymal Multipotent Stromal Cells of the Lungs in Pneumofibrosis

• Published in: Bulletin of experimental biology and medicine Vol. 154; no. 4; pp. 537 - 543
• Main Authors: Skurikhin, E. G., Khmelevskaya, E. S., Pershina, O. V., Ermakova, N. N., Krupin, V. A., Reztsova, A. M., Ermolaeva, L. A., Yakushina, V. D., Stepanova, I. E., Reztsova, V. M., Cherdyntseva, N. V., Stakheeva, M. N., Dygai, A. M.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-02-2013; Springer; Springer Nature B.V
• Subjects: Adipocytes - cytology; Animals; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Differentiation - physiology; Chondrocytes - cytology; Collagen; Fibroblasts - cytology; Fibrosis - pathology; Internal Medicine; Laboratory Medicine; Leukocytes, Mononuclear - cytology; Lung - cytology; Lung Diseases - pathology; Mesenchymal Stem Cells - cytology; Mice; Mice, Inbred C57BL; Pathology; Stem cells; lung fibrosis; differentiation of multipotent mesenchymal stromal cell; multipotent mesenchymal stromal cell
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-013-1995-6

We studied differentiation of multipotent mesenchymal stromal cells (MMSC) of the lungs of C57Bl/6 mice with bleomycin-induced pneumofibrosis. Adherent mononuclear cells found in mouse lungs demonstrated mesenchymal phenotype and expressed CD44, CD73, CD90, and CD106, but not CD31, CD34, and CD45. The cells with MMSC characteristics differentiate
in vitro
into various cells of stromal lines (chondrocytes, osteogenic cells, adipocytes, and fibroblasts). Bleomycin increased the growth rate of MMSC and selectively promoted their differentiation towards fibroblast cells.