Compatibility in the Biomphalaria glabrata/Echinostoma caproni model: Potential involvement of proteins from hemocytes revealed by a proteomic approach

• Published in: Acta tropica Vol. 98; no. 3; pp. 234 - 246
• Main Authors: Bouchut, A., Sautiere, P.E., Coustau, C., Mitta, G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-07-2006; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biodiversity; Biological and medical sciences; Biomphalaria; Biomphalaria - parasitology; Biomphalaria glabrata; Compatibility; Echinostoma; Echinostoma - physiology; Echinostoma caproni; Gene Expression Profiling; Gene Expression Regulation; General aspects; Hemocytes; Hemocytes - metabolism; Host–parasite interactions; Life Sciences; Medical sciences; Models, Biological; Molecular Sequence Data; Populations and Evolution; Proteins; Proteins - chemistry; Proteins - metabolism; Proteomic; Proteomics; Proteomics - methods; Research Support, Non-U.S. Gov't; Biomphalaria glabrata; Compatibility; Host–parasite interactions; Echinostoma caproni; Proteomic; Hemocytes; Plathelmintha; Gastropoda; Parasitosis; Trematoda; Protein; Infection; Pulmonata; Proteomics; Host-parasite interactions; Helmintha; Tropical medicine; Models; Invertebrata; Mollusca
• ISSN: 0001-706X; 1873-6254
• DOI: 10.1016/j.actatropica.2006.05.007

As an approach to investigate the suspected involvement of cellular factors in
Biomphalaria glabrata resistance/susceptibility to
Echinostoma caproni, we compared protein patterns from hemocytes collected from susceptible and resistant snails. This proteomic approach revealed that twelve hemocytic proteins exhibited significant differences in their apparent abundance. The genes corresponding to five of them were characterized by a combination of mass spectrometry and molecular cloning. They encode an aldolase, an intermediate filament protein, a cytidine deaminase, the ribosomal protein P1 and the histone H4. Furthermore, we investigated their expression in parasite-exposed or -unexposed snails. These last experiments revealed changes in transcript levels corresponding to intermediate filament and histone H4 proteins post-infection.