A Study on the Phytotoxic Potential of the Seasoning Herb Marjoram (Origanum majorana L.) Leaves

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 11; p. 3356
• Main Authors: Cala, Antonio, Salcedo, José R., Torres, Ascensión, Varela, Rosa M., Molinillo, José M. G., Macías, Francisco A.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 02-06-2021; MDPI
• Subjects: Acetic acid; Acetone; Analgesics; Antibiotics; bioassay; Bioassays; Biocompatibility; bioguided fractionation; Biological activity; Chlorophyll; Ethyl acetate; Flavonoids; Fractionation; Herbicides; Herbs; Leaves; marjoram; Monoterpenes; Natural products; Oils & fats; Origanum majorana; Origanum majorana L; Phytotoxicity; Plant extracts; spice; Spices; Toxic hazards; Weed control; wheat coleoptile
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26113356

In the search of new alternatives for weed control, spices appear as an option with great potential. They are rich in bioactive natural products and edible, which might minimize toxicity hazard. Marjoram (Origanum majorana L.) is an aromatic herb that has been widely employed as a seasoning herb in Mediterranean countries. Although marjoram boasts a plethora of therapeutic properties (painkiller, antibiotic, treatment for intestinal disorders, etc.), the potential for its extracts for weed control is still to be more thoroughly explored. In order to determine their phytotoxic potential, marjoram leaves were subjected to different bioguided extraction processes, using water, ethyl acetate, acetone or methanol. The most active extract (acetone) was sequentially fractionated to identify its most active compounds. This fractionation led to the isolation and identification of 25 compounds that were classified as monoterpenes, diterpenes or flavonoids. Among them, a new compound named majoradiol and several compounds are described in marjoram for the first time. The phytotoxicity of the major compounds to etiolated wheat coleoptiles was compared against that of the commercial herbicide (Logran®), with similar or higher activity in some cases. These results confirm the extraordinary potential of the extracts from this edible plant to develop safer and more environmentally friendly herbicides.