Amelioration of antioxidant potential, toxicity, and antihyperglycemic activity of Hippophae salicifolia D. Don leaf extracts in alloxan-induced diabetic rats

• Published in: 3 Biotech Vol. 9; no. 8; pp. 308 - 8
• Main Authors: Middha, Sushil Kumar, Usha, Talambedu, Basistha, Bharat Chandra, Goyal, Arvind Kumar
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-08-2019; Springer Nature B.V
• Subjects: Acute toxicity; Agriculture; Alloxan; Antioxidants; Bioinformatics; Biomaterials; Biotechnology; Body weight; Cancer Research; Chemistry; Chemistry and Materials Science; Diabetes; Diabetes mellitus; Glutathione; Glutathione peroxidase; Hippophae; Leaves; Lethal dose; Malondialdehyde; Original; Original Article; Oxidative stress; Peroxidase; Plant extracts; Reactive oxygen species; Rodents; Stem Cells; Superoxide dismutase; Toxicity testing; Antioxidants; Polyphenols; Toxicology; Flavonoids; Diabetes; Sea buckthorn; Hippophae salicifolia
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-019-1840-3

Efficacy of several plant extracts in the clinical research for modulating oxidative stress correlated with diabetes mellitus (DM) is well documented. In the present study, we investigated the in vitro antioxidant activity, toxicity, and anti-diabetic activity of methanolic extract of
Hippophae salicifolia
leaves in normal and alloxan-induced diabetic wistar rats.
H. salicifolia
leaves were found to be rich in antioxidants. The acute toxicity test of methanolic extract of
H. salicifolia
leaves revealed that the median lethal dose (LD
50
) was found to be 3.92 g/kg body weight in mice. Administration of
H. salicifolia
leaves at 200 mg/kg and 400 mg/kg in alloxan-induced diabetic rats illustrated significant reduction (22% and 39%, respectively) in fasting blood glucose compared to diabetic control. Both the doses were found to be effective when compared to diabetic rats. The
Hippophae-
treated diabetic rats showed significant increase in the endogenous antioxidant enzymes, superoxide dismutase (50% and 74%, respectively), glutathione peroxidase (57% and 41%, respectively) and decrease in malondialdehyde (33% and 15%, respectively) levels. These results suggested that the methanolic leaf extract of
H. salicifolia
enhanced the antioxidant defence against reactive oxygen species produced under hyperglycaemic conditions.