Formation of stable triplexes between purine RNA and pyrimidine oligodeoxyxylonucleotides

Hybridization properties of oligodeoxyxylonucleotides (OXNs) built from pyrimidine monomers with an inverted 3′‐OH group of the furanose have been studied using the gel mobility shift, UV melting and circular dichroism (CD) spectroscopy methods. Pyrimidine OXNs form triple helices with complementary...

Full description

Saved in:

Bibliographic Details
Published in:	Nucleic acids research Vol. 31; no. 14; pp. 4256 - 4263
Main Authors:	Ivanov, Sergei, Alekseev, Yakov, Bertrand, Jean‐Remi, Malvy, Claude, Gottikh, Marina B.
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 15-07-2003 Oxford Publishing Limited (England)
Subjects:	Animals Base Sequence Circular Dichroism DNA - chemistry DNA - genetics Hydrogen-Ion Concentration Mice Nucleic Acid Conformation Nucleic Acid Denaturation Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - genetics Protein Serine-Threonine Kinases - genetics Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins c-pim-1 Purines - chemistry Pyrimidines - chemistry RNA - chemistry RNA - genetics RNA, Messenger - chemistry RNA, Messenger - genetics Temperature Transcription, Genetic Xylose - chemistry
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Hybridization properties of oligodeoxyxylonucleotides (OXNs) built from pyrimidine monomers with an inverted 3′‐OH group of the furanose have been studied using the gel mobility shift, UV melting and circular dichroism (CD) spectroscopy methods. Pyrimidine OXNs form triple helices with complementary purine RNA in which one OXN is parallel and another is antiparallel with respect to the RNA target. Surprisingly, no duplex formation between the pyrimidine OXNs and purine RNAs is detected. The modified triplexes are stable at pH 7. Their thermal stability depends on the number of C(G‐C) triplets and, for G‐rich RNA sequences, it is comparable with the stability of native DNA–RNA duplexes. The CD spectra of triplexes formed by OXNs with purine RNA targets are similar to spectra of A‐type helices. A pyrimidine OXN having a clamp structure efficiently inhibits reverse transcription of murine pim‐1 mRNA in vitro mediated by the Mo‐MuLV reverse transcriptase.
Bibliography:	local:gkg443 ark:/67375/HXZ-M878Q0C6-C Received December 12, 2002; Revised March 7, 2003; Accepted April 30, 2003 To whom correspondence should be addressed. Tel: +33 142 11 5045; Fax: +33 142 11 5245; Email: jrb@igr.fr  This article is dedicated to the memory of our colleague, Prof. Claude Helene, who died on February 11, 2003. He was known nationally and internationally for his work on nucleic acids and more particularly on triple helices. He was always open to discussions with and advice to his colleagues. istex:99A08CB9539872B5D3E3FA2AD583A41EAC4CB5C0 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 To whom correspondence should be addressed. Tel: +33 142 11 5045; Fax: +33 142 11 5245; Email: jrb@igr.fr This article is dedicated to the memory of our colleague, Prof. Claude Helene, who died on February 11, 2003. He was known nationally and internationally for his work on nucleic acids and more particularly on triple helices. He was always open to discussions with and advice to his colleagues.
ISSN:	0305-1048 1362-4962 1362-4962
DOI:	10.1093/nar/gkg443