Epidermal label-retaining cells: background and recent applications

Epidermal label-retaining cells: background and recent applications

Epidermal label-retaining cells (LRC) can be identified by giving neonatal mice repeated injections of 3H-thymidine or 5-bromo-2'-deoxyuridine and then finding the cells that are still labelled in adulthood. Although label retention is simply a marker of the proliferative history of a cell, the...

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Bibliographic Details
Published in:The Journal of investigative dermatology symposium proceedings Vol. 9; no. 3; p. 196
Main Authors: Braun, Kristin M, Watt, Fiona M
Format: Journal Article
Language:English
Published: United States 01-09-2004
Subjects:
Animals
Epidermis - cytology
Epidermis - physiology
Humans
Stem Cells - cytology
Stem Cells - physiology
Thymidine - pharmacokinetics
Tritium
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Summary:Epidermal label-retaining cells (LRC) can be identified by giving neonatal mice repeated injections of 3H-thymidine or 5-bromo-2'-deoxyuridine and then finding the cells that are still labelled in adulthood. Although label retention is simply a marker of the proliferative history of a cell, there is, nevertheless, evidence that it is a characteristic of epidermal stem cells. Here we review the early literature on LRC and then highlight two recent applications. We describe how LRC can be visualized by whole-mount labelling of the epidermis and how purified LRC can be used to screen for markers of the epidermal stem cell compartment.
ISSN:1087-0024
DOI:10.1111/j.1087-0024.2004.09313.x