Image Processing Techniques for Improving Quality of 3D Profile in Digital Holographic Microscopy Using Deep Learning Algorithm

• Published in: Sensors (Basel, Switzerland) Vol. 24; no. 6; p. 1950
• Main Authors: Kim, Hyun-Woo, Cho, Myungjin, Lee, Min-Chul
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19-03-2024; MDPI
• Subjects: Algorithms; Data mining; Deep learning; Digital Holographic Microscopy (DHM); Fourier transforms; Image processing; Imaging systems; Improved Denoising Diffusion Probabilistic Models (IDDPM); Methods; Microscope and microscopy; Microscopy; Noise control; noise filtering; Technology application; Three dimensional imaging; Improved Denoising Diffusion Probabilistic Models (IDDPM); Digital Holographic Microscopy (DHM); noise filtering
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s24061950

Digital Holographic Microscopy (DHM) is a 3D imaging technology widely applied in biology, microelectronics, and medical research. However, the noise generated during the 3D imaging process can affect the accuracy of medical diagnoses. To solve this problem, we proposed several frequency domain filtering algorithms. However, the filtering algorithms we proposed have a limitation in that they can only be applied when the distance between the direct current (DC) spectrum and sidebands are sufficiently far. To address these limitations, among the proposed filtering algorithms, the HiVA algorithm and deep learning algorithm, which effectively filter by distinguishing between noise and detailed information of the object, are used to enable filtering regardless of the distance between the DC spectrum and sidebands. In this paper, a combination of deep learning technology and traditional image processing methods is proposed, aiming to reduce noise in 3D profile imaging using the Improved Denoising Diffusion Probabilistic Models (IDDPM) algorithm.