Mitochondrial nanomotion measured by optical microscopy
Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also f...
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| Published in: | Frontiers in microbiology Vol. 14; p. 1133773 |
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| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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23-03-2023
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| Abstract | Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free. |
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| AbstractList | Nanometric scale size oscillations seem to be a fundamental feature of all living organisms on Earth. Their detection usually requires complex and very sensitive devices. However, some recent studies demonstrated that very simple optical microscopes and dedicated image processing software can also fulfill this task. This novel technique, termed as optical nanomotion detection (ONMD), was recently successfully used on yeast cells to conduct rapid antifungal sensitivity tests. In this study, we demonstrate that the ONMD method can monitor motile sub-cellular organelles, such as mitochondria. Here, mitochondrial isolates (from HEK 293 T and Jurkat cells) undergo predictable motility when viewed by ONMD and triggered by mitochondrial toxins, citric acid intermediates, and dietary and bacterial fermentation products (short-chain fatty acids) at various doses and durations. The technique has superior advantages compared to classical methods since it is rapid, possesses a single organelle sensitivity, and is label- and attachment-free. |
| Author | Kalauzi, Aleksandar MacFabe, Derrick F Kasas, Sandor Parmar, Priyanka Villalba, Maria Ines Bartolić, Dragana Radotić, Ksenija Horii Huber, Alexandre Seiji Willaert, Ronnie Guy |
| AuthorAffiliation | 4 Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), Research Group Structural Biology Brussels, Vrije Universiteit Brussel , Brussels , Belgium 2 International Joint Research Group VUB-EPFL NanoBiotechnology and NanoMedicine (NANO), Vrije Universiteit Brussel and École Polytechnique Fédérale de Lausanne (EPFL) , Lausanne , Switzerland 6 Department of Microbiology, Faculty of Medicine, Centre of Healthy Eating and Food Innovation (HEFI), Maastricht University , Maastricht , Netherlands 7 Centre Universitaire Romand de Médecine Légale, UFAM, University of Lausanne , Lausanne , Switzerland 3 Institute for Multidisciplinary Research, University of Belgrade , Belgrade , Serbia 5 Kilee Patchell-Evans Autism Research Group , London, ON , Canada 1 Laboratory of Biological Electron Microscopy, École Polytechnique Fédérale de Lausanne (EPFL) and University of Lausanne (UNIL) , Lausanne , Switzerland |
| AuthorAffiliation_xml | – name: 1 Laboratory of Biological Electron Microscopy, École Polytechnique Fédérale de Lausanne (EPFL) and University of Lausanne (UNIL) , Lausanne , Switzerland – name: 6 Department of Microbiology, Faculty of Medicine, Centre of Healthy Eating and Food Innovation (HEFI), Maastricht University , Maastricht , Netherlands – name: 4 Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), Research Group Structural Biology Brussels, Vrije Universiteit Brussel , Brussels , Belgium – name: 7 Centre Universitaire Romand de Médecine Légale, UFAM, University of Lausanne , Lausanne , Switzerland – name: 2 International Joint Research Group VUB-EPFL NanoBiotechnology and NanoMedicine (NANO), Vrije Universiteit Brussel and École Polytechnique Fédérale de Lausanne (EPFL) , Lausanne , Switzerland – name: 5 Kilee Patchell-Evans Autism Research Group , London, ON , Canada – name: 3 Institute for Multidisciplinary Research, University of Belgrade , Belgrade , Serbia |
| Author_xml | – sequence: 1 givenname: Priyanka surname: Parmar fullname: Parmar, Priyanka organization: Laboratory of Biological Electron Microscopy, École Polytechnique Fédérale de Lausanne (EPFL) and University of Lausanne (UNIL), Lausanne, Switzerland – sequence: 2 givenname: Maria Ines surname: Villalba fullname: Villalba, Maria Ines organization: International Joint Research Group VUB-EPFL NanoBiotechnology and NanoMedicine (NANO), Vrije Universiteit Brussel and École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland – sequence: 3 givenname: Alexandre Seiji surname: Horii Huber fullname: Horii Huber, Alexandre Seiji organization: Laboratory of Biological Electron Microscopy, École Polytechnique Fédérale de Lausanne (EPFL) and University of Lausanne (UNIL), Lausanne, Switzerland – sequence: 4 givenname: Aleksandar surname: Kalauzi fullname: Kalauzi, Aleksandar organization: Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia – sequence: 5 givenname: Dragana surname: Bartolić fullname: Bartolić, Dragana organization: Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia – sequence: 6 givenname: Ksenija surname: Radotić fullname: Radotić, Ksenija organization: Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia – sequence: 7 givenname: Ronnie Guy surname: Willaert fullname: Willaert, Ronnie Guy organization: Alliance Research Group VUB-UGent NanoMicrobiology (NAMI), Research Group Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium – sequence: 8 givenname: Derrick F surname: MacFabe fullname: MacFabe, Derrick F organization: Department of Microbiology, Faculty of Medicine, Centre of Healthy Eating and Food Innovation (HEFI), Maastricht University, Maastricht, Netherlands – sequence: 9 givenname: Sandor surname: Kasas fullname: Kasas, Sandor organization: Centre Universitaire Romand de Médecine Légale, UFAM, University of Lausanne, Lausanne, Switzerland |
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| Cites_doi | 10.1371/journal.pone.0044610 10.1016/j.ebiom.2021.103293 10.1021/acsnano.5b05944 10.3402/mehd.v26.28177 10.1093/carcin/bgt480 10.1073/pnas.1522556113 10.1038/nnano.2013.120 10.1007/s11010-013-1781-9 10.3389/fendo.2020.00025 10.1111/jnc.14928 10.1016/j.tcsw.2019.100021 10.1128/AAC.02194-18 10.1016/j.celrep.2020.01.078 10.1002/jdn.10011 10.1038/tp.2016.189 10.1038/s41565-022-01111-6 10.5194/ms-8-23-2017 10.1002/smll.201702671 10.1007/978-1-4939-7831-1_15 10.3389/fimmu.2017.01670 10.1126/sciadv.aba3139 10.1016/j.mito.2020.06.008 10.3791/52076 10.3390/ph14080767 10.1136/bmjdrc-2021-002336 10.3390/fermentation6010028 10.1523/JNEUROSCI.23-34-10756.2003 10.1073/pnas.1415348112 |
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| Copyright | Copyright © 2023 Parmar, Villalba, Horii Huber, Kalauzi, Bartolić, Radotić, Willaert, MacFabe and Kasas. Copyright © 2023 Parmar, Villalba, Horii Huber, Kalauzi, Bartolić, Radotić, Willaert, MacFabe and Kasas. 2023 Parmar, Villalba, Horii Huber, Kalauzi, Bartolić, Radotić, Willaert, MacFabe and Kasas |
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| Keywords | rotenone optical nanomotion metabolic substrates mitochondria short chain fatty acids |
| Language | English |
| License | Copyright © 2023 Parmar, Villalba, Horii Huber, Kalauzi, Bartolić, Radotić, Willaert, MacFabe and Kasas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Matthew Cabeen, Oklahoma State University, United States; Mar Alvarez, Institute of Microelectronics of Barcelona (CSIC), Spain; Ferran Pujol-Vila, National Microelectronics Center (CSIC), Spain Edited by: Vijay Pancholi, The Ohio State University, United States This article was submitted to Microbial Physiology and Metabolism, a section of the journal Frontiers in Microbiology These authors have contributed equally to this work and share first authorship These authors have contributed equally to this work and share last authorship |
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| SubjectTerms | metabolic substrates Microbiology mitochondria optical nanomotion rotenone short chain fatty acids |
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| Title | Mitochondrial nanomotion measured by optical microscopy |
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