Detection of variant infectious bronchitis viruses in broiler flocks in Libya

• Published in: International journal of veterinary science and medicine Vol. 2; no. 1; pp. 78 - 82
• Main Authors: Awad, Faez, Baylis, Matthew, Ganapathy, Kannan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-06-2014; Taylor & Francis; Taylor & Francis Group
• Subjects: Chicken; EXO, Exonuclease; FTA, Flinders Technology Associates; IBD, infectious bursal disease; IBV, infectious bronchitis virus; Libya; ND, Newcastle disease; OP, oropharyngeal swab; RNA, ribonucleic acid; RT-PCR, reverse transcription-polymerase chain reaction; SAP, shrimp alkaline phosphatase; Variant infectious bronchitis virus; OP; Variant infectious bronchitis virus; FTA; SAP; RNA; ND; RT-PCR; IBD; Chicken; IBV; EXO; Libya
• ISSN: 2314-4599; 2314-4599
• DOI: 10.1016/j.ijvsm.2014.01.001

A number of broiler flocks with respiratory disease and high mortality in five broiler farms in Libya were sampled for detection of infectious bronchitis virus (IBV). Twelve IBV strains from these farms were detected by reverse transcription polymerase chain reaction (RT-PCR) and differentiated by nucleotide sequencing of the hypervariable region of the S1 gene. A pair-wise comparison of the sequences showed two distinctive patterns. Those from farms 1, 2, 4 and 5, formed a separate cluster with 94–99% relatedness to the Egyptian IBV strains CK/Eg/BSU-2/2011, CK/Eg/BSU-3/2011 and Eg/1212B. Sequences from the farm 3 formed another cluster with 100% relatedness to Eg/CLEVB-2/IBV/012 and IS/1494/06. This appears to be the first report on the co-circulation these variant IBVs in Libya.