Proteomic analysis of Escherichia coli with experimentally induced resistance to piperacillin/tazobactam

• Published in: Research in microbiology Vol. 161; no. 4; pp. 268 - 275
• Main Authors: Valéria dos Santos, Kênia, Diniz, Cláudio Galuppo, de Castro Veloso, Luciano, Monteiro de Andrade, Hélida, da Silva Giusta, Mario, da Fonseca Pires, Simone, Santos, Agenor Valadares, Morais Apolônio, Ana Carolina, Roque de Carvalho, Maria Auxiliadora, de Macêdo Farias, Luiz
• Format: Journal Article
• Language: English
• Published: Issy-les-Moulineaux Elsevier SAS 01-05-2010; Elsevier Masson
• Subjects: 2-D DIGE; Amino acids; Analytical, structural and metabolic biochemistry; Anti-Bacterial Agents - pharmacology; Bacteriology; Biological and medical sciences; Comparative proteome; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Two-Dimensional; Escherichia coli; Escherichia coli - chemistry; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Mass Spectrometry; Microbiology; Miscellaneous; Molecular Sequence Data; Penicillanic Acid - analogs & derivatives; Penicillanic Acid - pharmacology; Piperacillin - pharmacology; Piperacillin/tazobactam; Proteins; Proteomics; Resistance; Resistance; 2-D DIGE; Comparative proteome; Escherichia coli; Piperacillin/tazobactam; Enzyme; β-Lactams; Enzyme inhibitor; Proteome; β-Lactamase; Induced resistance; Penicillin derivatives; Antibiotic; Tazobactam; Piperacillin; Proteomics; Bacteria; Hydrolases; Antibacterial agent; Enterobacteriaceae
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/j.resmic.2010.03.006

The worldwide emergence of antibiotic-resistant bacteria poses a serious threat to human health. In addition to the difficulties in controlling infectious diseases, the phenotype of resistance can generate metabolic changes which, in turn, can interfere with host–pathogen interactions. The aim of the present study was to identify changes in the subproteome of a laboratory-derived piperacillin/tazobactam-resistant strain of
Escherichia coli (minimal inhibitory concentration [MIC] = 128 mg/L) as compared with its susceptible wild-type strain
E. coli ATCC 25922 (MIC = 2 mg/L) using 2-D fluorescence difference gel electrophoresis (2D-DIGE) followed by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight (MALDI-TOF/TOF MS). In the resistant strain, a total of 12 protein species were increased in abundance relative to the wild-type strain, including those related to bacterial virulence, antibiotic resistance and DNA protection during stress. Fourteen proteins were increased in abundance in the wild-type strain compared to the resistant strain, including those involved in glycolysis, protein biosynthesis, pentose-phosphate shunt, amino acid transport, cell division and oxidative stress response. In conclusion, our data show overall changes in the subproteome of the piperacillin/tazobactam-resistant strain, reporting for the first time the potential role of a multidrug efflux pump system in
E. coli resistance to piperacillin/tazobactam.