Mycobacterial IHF is a highly dynamic nucleoid-associated protein that assists HupB in organizing chromatin
Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration host factor (mIHF), a...
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| Published in: | Frontiers in microbiology Vol. 14; p. 1146406 |
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| Abstract | Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration host factor (mIHF), are found in mycobacteria. Despite their importance for the pathogenicity and/or survival of tubercle bacilli, the role of these proteins in mycobacterial chromosome organization remains unknown. Here, we used various approaches, including super-resolution microscopy, to perform a comprehensive analysis of the roles of HupB and mIHF in chromosome organization. We report that HupB is a structural agent that maintains chromosome integrity on a local scale, and that the lack of this protein alters chromosome morphology. In contrast, mIHF is a highly dynamic protein that binds DNA only transiently, exhibits susceptibility to the chromosomal DNA topology changes and whose depletion leads to the growth arrest of tubercle bacilli. Additionally, we have shown that depletion of
integration host factor (msIHF) leads to chromosome shrinkage and replication inhibition. |
|---|---|
| AbstractList | Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration host factor (mIHF), are found in mycobacteria. Despite their importance for the pathogenicity and/or survival of tubercle bacilli, the role of these proteins in mycobacterial chromosome organization remains unknown. Here, we used various approaches, including super-resolution microscopy, to perform a comprehensive analysis of the roles of HupB and mIHF in chromosome organization. We report that HupB is a structural agent that maintains chromosome integrity on a local scale, and that the lack of this protein alters chromosome morphology. In contrast, mIHF is a highly dynamic protein that binds DNA only transiently, exhibits susceptibility to the chromosomal DNA topology changes and whose depletion leads to the growth arrest of tubercle bacilli. Additionally, we have shown that depletion of Mycobacterium smegmatis integration host factor (msIHF) leads to chromosome shrinkage and replication inhibition. Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration host factor (mIHF), are found in mycobacteria. Despite their importance for the pathogenicity and/or survival of tubercle bacilli, the role of these proteins in mycobacterial chromosome organization remains unknown. Here, we used various approaches, including super-resolution microscopy, to perform a comprehensive analysis of the roles of HupB and mIHF in chromosome organization. We report that HupB is a structural agent that maintains chromosome integrity on a local scale, and that the lack of this protein alters chromosome morphology. In contrast, mIHF is a highly dynamic protein that binds DNA only transiently, exhibits susceptibility to the chromosomal DNA topology changes and whose depletion leads to the growth arrest of tubercle bacilli. Additionally, we have shown that depletion of integration host factor (msIHF) leads to chromosome shrinkage and replication inhibition. Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration host factor (mIHF), are found in mycobacteria. Despite their importance for the pathogenicity and/or survival of tubercle bacilli, the role of these proteins in mycobacterial chromosome organization remains unknown. Here, we used various approaches, including super-resolution microscopy, to perform a comprehensive analysis of the roles of HupB and mIHF in chromosome organization. We report that HupB is a structural agent that maintains chromosome integrity on a local scale, and that the lack of this protein alters chromosome morphology. In contrast, mIHF is a highly dynamic protein that binds DNA only transiently, exhibits susceptibility to the chromosomal DNA topology changes and whose depletion leads to the growth arrest of tubercle bacilli. Additionally, we have shown that depletion of Mycobacterium smegmatis integration host factor (msIHF) leads to chromosome shrinkage and replication inhibition. |
| Author | Giacomelli, Giacomo Drużka, Karolina Trojanowski, Damian Bramkamp, Marc Łebkowski, Tomasz Hołówka, Joanna Zakrzewska-Czerwińska, Jolanta Broda, Natalia Feddersen, Helge Makowski, Łukasz |
| AuthorAffiliation | 1 Department of Molecular Microbiology, University of Wrocław , Wrocław , Poland 2 Institute for General Microbiology, Christian-Albrechts-University , Kiel , Germany |
| AuthorAffiliation_xml | – name: 1 Department of Molecular Microbiology, University of Wrocław , Wrocław , Poland – name: 2 Institute for General Microbiology, Christian-Albrechts-University , Kiel , Germany |
| Author_xml | – sequence: 1 givenname: Joanna surname: Hołówka fullname: Hołówka, Joanna organization: Department of Molecular Microbiology, University of Wrocław, Wrocław, Poland – sequence: 2 givenname: Tomasz surname: Łebkowski fullname: Łebkowski, Tomasz organization: Department of Molecular Microbiology, University of Wrocław, Wrocław, Poland – sequence: 3 givenname: Helge surname: Feddersen fullname: Feddersen, Helge organization: Institute for General Microbiology, Christian-Albrechts-University, Kiel, Germany – sequence: 4 givenname: Giacomo surname: Giacomelli fullname: Giacomelli, Giacomo organization: Institute for General Microbiology, Christian-Albrechts-University, Kiel, Germany – sequence: 5 givenname: Karolina surname: Drużka fullname: Drużka, Karolina organization: Department of Molecular Microbiology, University of Wrocław, Wrocław, Poland – sequence: 6 givenname: Łukasz surname: Makowski fullname: Makowski, Łukasz organization: Department of Molecular Microbiology, University of Wrocław, Wrocław, Poland – sequence: 7 givenname: Damian surname: Trojanowski fullname: Trojanowski, Damian organization: Department of Molecular Microbiology, University of Wrocław, Wrocław, Poland – sequence: 8 givenname: Natalia surname: Broda fullname: Broda, Natalia organization: Department of Molecular Microbiology, University of Wrocław, Wrocław, Poland – sequence: 9 givenname: Marc surname: Bramkamp fullname: Bramkamp, Marc organization: Institute for General Microbiology, Christian-Albrechts-University, Kiel, Germany – sequence: 10 givenname: Jolanta surname: Zakrzewska-Czerwińska fullname: Zakrzewska-Czerwińska, Jolanta organization: Department of Molecular Microbiology, University of Wrocław, Wrocław, Poland |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36960278$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright © 2023 Hołówka, Łebkowski, Feddersen, Giacomelli, Drużka, Makowski, Trojanowski, Broda, Bramkamp and Zakrzewska-Czerwińska. Copyright © 2023 Hołówka, Łebkowski, Feddersen, Giacomelli, Drużka, Makowski, Trojanowski, Broda, Bramkamp and Zakrzewska-Czerwińska. 2023 Hołówka, Łebkowski, Feddersen, Giacomelli, Drużka, Makowski, Trojanowski, Broda, Bramkamp and Zakrzewska-Czerwińska |
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| Keywords | mycobacterial IHF nucleoid-associated protein HupB mycobacteria bacterial chromosome compaction |
| Language | English |
| License | Copyright © 2023 Hołówka, Łebkowski, Feddersen, Giacomelli, Drużka, Makowski, Trojanowski, Broda, Bramkamp and Zakrzewska-Czerwińska. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Valakunja Nagaraja, Indian Institute of Science (IISc), India; Remus T. Dame, Leiden University, Netherlands These authors have contributed equally to this work Edited by: Tomohiro Shimada, Meiji University, Japan This article was submitted to Evolutionary and Genomic Microbiology, a section of the journal Frontiers in Microbiology |
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