Electron Capture, Collision-Induced, and Electron Capture-Collision Induced Dissociation in Q-TOF

• Published in: Journal of the American Society for Mass Spectrometry Vol. 22; no. 4; pp. 607 - 611
• Main Authors: Voinov, Valery G., Deinzer, Max L., Beckman, Joseph S., Barofsky, Douglas F.
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01-04-2011; Springer Nature B.V
• Subjects: Analytical Chemistry; Beta decay; Bioinformatics; Biotechnology; Chemistry; Chemistry and Materials Science; Electron capture; Electrons; Ions; Mass spectra; Mass spectrometry; Oligopeptides - chemistry; Organic Chemistry; Peptides; Proteomics; Radio frequency; Retrofitting; Short Communication; Tandem Mass Spectrometry - methods; Electron capture dissociation; A radio-frequency-free electromagnetostatic cell
• ISSN: 1044-0305; 1879-1123
• DOI: 10.1007/s13361-010-0072-x

Recently, we demonstrated that a radio-frequency-free electromagnetostatic (rf-free EMS) cell could be retrofitted into a triple quad mass spectrometer to allow electron-capture dissociation (ECD) without the aid of cooling gas or phase-specific electron injection into the cell (Voinov et al., Rapid Commun Mass Spectrom 22, 3087–3088,
2008
; Voinov et al., Anal Chem 81, 1238–1243,
2009
). Subsequently, we used our rf-free EMS cell in the same instrument platform to demonstrate ECD occurring in the same space and at the same time with collision-induced dissociation (CID) to produce golden pairs and even triplets from peptides (Voinov et al., Rapid Commun Mass Spectrom 23, 3028–3030,
2009
). In this report, we demonstrate that ECD and CID product-ion mass spectra can be recorded at high resolution with flexible control of fragmentation processes using a newly designed cell installed in a hybrid Q-TOF tandem mass spectrometer.