Performance of the HSV OligoGen kit for the diagnosis of herpes simplex virus type 1 and 2

• Published in: Diagnostic microbiology and infectious disease Vol. 85; no. 3; pp. 315 - 317
• Main Authors: Parra-Sánchez, Manuel, López, Ana Marcuello, García-Rey, Silvia, Breval, Ismail Zakariya-Yousef, Martínez, Samuel Bernal, Rodríguez, Isabel Pueyo, Martín-Mazuelos, Estrella, Folía, José Carlos Palomares
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2016
• Subjects: Adult; Diagnostic test; Female; Genital herpes infection; Herpes Genitalis - diagnosis; Herpes Simplex - diagnosis; Herpes simplex virus 1; Herpes simplex virus 2; Herpesvirus 1, Human - genetics; Herpesvirus 1, Human - isolation & purification; Herpesvirus 2, Human - genetics; Herpesvirus 2, Human - isolation & purification; HSV-1; HSV-2; Humans; Infectious Disease; Internal Medicine; Male; Molecular Diagnostic Techniques - methods; Predictive Value of Tests; Prospective Studies; Sensitivity and Specificity; Sexually transmitted infections; Spain; Young Adult; HSV-1; HSV-2; genital herpes infection; diagnostic test; Sexually transmitted infections; Diagnostic test; Genital herpes infection
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2016.04.019

Abstract PCR methods are nowadays between the most rapid and sensitive methods for screening and diagnosing herpes simplex virus (HSV) type 1 and 2. The aim of this study was to analyze the reliability, accuracy, and usefulness of the new assay HSV OligoGen kit in comparison with the Roche LightCycler® HSV ½ Qual Kit assay for the detection of HSV in clinical samples. For this analysis, a prospective study was designed for detection of HSV-1 and HSV-2 including 110 ulcer specimens, 48 urine, 48 endocervical, 43 cerebral spinal fluids, 4 urethral and 3 pharyngeal swabs that were sent from a regional STI clinic or an Intensive Clinical Unit, both in Seville, Spain. In comparison to the Roche LightCycler® HSV ½ Qual Kit assay, sensitivity, specificity, positive and negative predicative values, and kappa value for HSV detection using the HSV OligoGen kit were 96.2%, 100%, 100%, 98.3%, and 0.97 for HSV-1, respectively. For HSV-2, the corresponding values were 98.3%, 100%, 100%, 99.5%, and 0.98, respectively. Statistical data obtained in this study confirms the usefulness and reliable results of this new assay.