Serum profile of pro- and anti-inflammatory cytokines in rats following implantation of low-temperature plasma-modified titanium plates

• Published in: Journal of materials science. Materials in medicine Vol. 23; no. 5; pp. 1299 - 1307
• Main Authors: Walschus, Uwe, Hoene, Andreas, Patrzyk, Maciej, Finke, Birgit, Polak, Martin, Lucke, Silke, Nebe, Barbara, Schroeder, Karsten, Podbielski, Andreas, Wilhelm, Lutz, Schlosser, Michael
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-05-2012; Springer; Springer Nature B.V
• Subjects: Animals; Anti-Inflammatory Agents - blood; Anti-Inflammatory Agents - metabolism; Biocompatibility; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Ceramics; Chemistry and Materials Science; Coated Materials, Biocompatible - pharmacology; Composites; Curing Lights, Dental; Cytokines; Cytokines - blood; Cytokines - metabolism; Equipment and Supplies - adverse effects; Glass; Implantation; Inflammation Mediators - blood; Inflammation Mediators - metabolism; Inflammatory diseases; Low temperature physics; Male; Materials Science; Medical sciences; Metabolome - drug effects; Natural Materials; Plasma; Plasma Gases - chemistry; Plasma Gases - pharmacology; Polymer Sciences; Polymerization; Prosthesis Implantation - adverse effects; Prosthesis Implantation - rehabilitation; Rats; Rats, Inbred Lew; Regenerative Medicine/Tissue Engineering; Rodents; Serums; Surface Properties; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments; Temperature effects; Thin Films; Titanium; Titanium - chemistry; Titanium - pharmacology; Phosphorylated Focal Adhesion Kinase; Natural Killer Cell; Serum Cytokine Level; Plasma Polymerize; Middle Phase; Rat; Rodentia; Antiinflammatory agent; Cytokine; Implantation; Plate; Vertebrata; Mammalia; Cold plasma; Animal; Titanium; Serum; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-012-4600-z

Surface modification of Titanium (Ti) by low-temperature plasma influences cell-material interactions. Therefore, this study aimed at examining serum cytokine levels and associations after intramuscular implantation (
n
 = 8 rats/group) of Ti-plates with Plasma Polymerized Allyl Amine (Ti-PPAAm), Plasma Polymerized Acrylic Acid (Ti-PPAAc), and without such layers (Ti-Controls). Pro-inflammatory (IL-2, IFNγ, IL-6) and anti-inflammatory (IL-4, IL-10, IL-13) cytokines were measured weekly for 56 days. Ti-PPAAm caused increased IL-2 (d7-14, d35), increased IFNγ (d35) and decreased IL-10 (d35, d49-56). Ti-PPAAc induced divergent anti-inflammatory cytokine changes with increased IL-4 (d28-56) and decreased IL-10 (d42-56). Ti-Controls elicited increased IL-2 (d42) and IFNγ (d35-42, d56). IL-6 was not detected and IL-13 only in three samples, thus they do not influence the response against these Ti implants. Correlation analysis revealed surface-dependent associations between cytokines indicating the involvement of different inflammatory cell populations. Concluding, different plasma modifications induce specific serum cytokine profiles and associations indicating distinct inflammatory responses.