Role of peptidoglycan recycling enzymes AmpD and AnmK in Acinetobacter baumannii virulence features
is an important causative agent of hospital acquired infections. In addition to acquired resistance to many currently-available antibiotics, it is intrinsically resistant to fosfomycin. It has previously been shown that AmpD and AnmK contribute to intrinsic fosfomycin resistance in due to their invo...
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| Published in: | Frontiers in cellular and infection microbiology Vol. 12; p. 1064053 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
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| Abstract | is an important causative agent of hospital acquired infections. In addition to acquired resistance to many currently-available antibiotics, it is intrinsically resistant to fosfomycin. It has previously been shown that AmpD and AnmK contribute to intrinsic fosfomycin resistance in
due to their involvement in the peptidoglycan recycling pathway. However, the role that these two enzymes play in the fitness and virulence of
has not been studied. The aim of this study was to characterize several virulence-related phenotypic traits in
mutants lacking AmpD and AnmK. Specifically, cell morphology, peptidoglycan thickness, membrane permeability, growth under iron-limiting conditions, fitness, resistance to disinfectants and antimicrobial agents, twitching motility and biofilm formation of the mutant strains
ATCC 17978 Δ
::Kan and Δ
::Kan were compared to the wild type strain. Our results demonstrate that bacterial growth and fitness of both mutants were compromised, especially in the Δ
::Kan mutant. In addition, biofilm formation was decreased by up to 69%, whereas twitching movement was reduced by about 80% in both mutants. These results demonstrate that, in addition to increased susceptibility to fosfomycin, alteration of the peptidoglycan recycling pathway affects multiple aspects related to virulence. Inhibition of these enzymes could be explored as a strategy to develop novel treatments for
in the future. Furthermore, this study establishes a link between intrinsic fosfomycin resistance mechanisms and bacterial fitness and virulence traits. |
|---|---|
| AbstractList | Acinetobacter baumannii is an important causative agent of hospital acquired infections. In addition to acquired resistance to many currently-available antibiotics, it is intrinsically resistant to fosfomycin. It has previously been shown that AmpD and AnmK contribute to intrinsic fosfomycin resistance in A. baumannii due to their involvement in the peptidoglycan recycling pathway. However, the role that these two enzymes play in the fitness and virulence of A. baumannii has not been studied. The aim of this study was to characterize several virulence-related phenotypic traits in A. baumannii mutants lacking AmpD and AnmK. Specifically, cell morphology, peptidoglycan thickness, membrane permeability, growth under iron-limiting conditions, fitness, resistance to disinfectants and antimicrobial agents, twitching motility and biofilm formation of the mutant strains A. baumannii ATCC 17978 ΔampD::Kan and ΔanmK::Kan were compared to the wild type strain. Our results demonstrate that bacterial growth and fitness of both mutants were compromised, especially in the ΔampD::Kan mutant. In addition, biofilm formation was decreased by up to 69%, whereas twitching movement was reduced by about 80% in both mutants. These results demonstrate that, in addition to increased susceptibility to fosfomycin, alteration of the peptidoglycan recycling pathway affects multiple aspects related to virulence. Inhibition of these enzymes could be explored as a strategy to develop novel treatments for A. baumannii in the future. Furthermore, this study establishes a link between intrinsic fosfomycin resistance mechanisms and bacterial fitness and virulence traits. Acinetobacter baumannii is an important causative agent of hospital acquired infections. In addition to acquired resistance to many currently-available antibiotics, it is intrinsically resistant to fosfomycin. It has previously been shown that AmpD and AnmK contribute to intrinsic fosfomycin resistance in A. baumannii due to their involvement in the peptidoglycan recycling pathway. However, the role that these two enzymes play in the fitness and virulence of A. baumannii has not been studied. The aim of this study was to characterize several virulence-related phenotypic traits in A. baumannii mutants lacking AmpD and AnmK. Specifically, cell morphology, peptidoglycan thickness, membrane permeability, growth under iron-limiting conditions, fitness, resistance to disinfectants and antimicrobial agents, twitching motility and biofilm formation of the mutant strains A. baumannii ATCC 17978 Δ ampD ::Kan and Δ anmK ::Kan were compared to the wild type strain. Our results demonstrate that bacterial growth and fitness of both mutants were compromised, especially in the Δ ampD ::Kan mutant. In addition, biofilm formation was decreased by up to 69%, whereas twitching movement was reduced by about 80% in both mutants. These results demonstrate that, in addition to increased susceptibility to fosfomycin, alteration of the peptidoglycan recycling pathway affects multiple aspects related to virulence. Inhibition of these enzymes could be explored as a strategy to develop novel treatments for A. baumannii in the future. Furthermore, this study establishes a link between intrinsic fosfomycin resistance mechanisms and bacterial fitness and virulence traits. is an important causative agent of hospital acquired infections. In addition to acquired resistance to many currently-available antibiotics, it is intrinsically resistant to fosfomycin. It has previously been shown that AmpD and AnmK contribute to intrinsic fosfomycin resistance in due to their involvement in the peptidoglycan recycling pathway. However, the role that these two enzymes play in the fitness and virulence of has not been studied. The aim of this study was to characterize several virulence-related phenotypic traits in mutants lacking AmpD and AnmK. Specifically, cell morphology, peptidoglycan thickness, membrane permeability, growth under iron-limiting conditions, fitness, resistance to disinfectants and antimicrobial agents, twitching motility and biofilm formation of the mutant strains ATCC 17978 Δ ::Kan and Δ ::Kan were compared to the wild type strain. Our results demonstrate that bacterial growth and fitness of both mutants were compromised, especially in the Δ ::Kan mutant. In addition, biofilm formation was decreased by up to 69%, whereas twitching movement was reduced by about 80% in both mutants. These results demonstrate that, in addition to increased susceptibility to fosfomycin, alteration of the peptidoglycan recycling pathway affects multiple aspects related to virulence. Inhibition of these enzymes could be explored as a strategy to develop novel treatments for in the future. Furthermore, this study establishes a link between intrinsic fosfomycin resistance mechanisms and bacterial fitness and virulence traits. |
| Author | López-Siles, Mireia McConnell, Michael J Tajuelo, Ana Terrón, María C |
| AuthorAffiliation | 4 Serra Húnter Fellow, Microbiology of Intestinal Diseases, Biology Department, Universitat de Girona , Girona , Spain 3 Electron Microscopy Unit, Scientific-Technical Central Units, Instituto de Salud Carlos III (ISCIII) , Madrid , Spain 2 Universidad Nacional de Educación a Distancia (UNED) , Madrid , Spain 1 Intrahospital Infections Laboratory, Instituto de Salud Carlos III (ISCIII), National Centre for Microbiology , Madrid , Spain |
| AuthorAffiliation_xml | – name: 2 Universidad Nacional de Educación a Distancia (UNED) , Madrid , Spain – name: 1 Intrahospital Infections Laboratory, Instituto de Salud Carlos III (ISCIII), National Centre for Microbiology , Madrid , Spain – name: 3 Electron Microscopy Unit, Scientific-Technical Central Units, Instituto de Salud Carlos III (ISCIII) , Madrid , Spain – name: 4 Serra Húnter Fellow, Microbiology of Intestinal Diseases, Biology Department, Universitat de Girona , Girona , Spain |
| Author_xml | – sequence: 1 givenname: Ana surname: Tajuelo fullname: Tajuelo, Ana organization: Universidad Nacional de Educación a Distancia (UNED), Madrid, Spain – sequence: 2 givenname: María C surname: Terrón fullname: Terrón, María C organization: Electron Microscopy Unit, Scientific-Technical Central Units, Instituto de Salud Carlos III (ISCIII), Madrid, Spain – sequence: 3 givenname: Mireia surname: López-Siles fullname: López-Siles, Mireia organization: Serra Húnter Fellow, Microbiology of Intestinal Diseases, Biology Department, Universitat de Girona, Girona, Spain – sequence: 4 givenname: Michael J surname: McConnell fullname: McConnell, Michael J organization: Intrahospital Infections Laboratory, Instituto de Salud Carlos III (ISCIII), National Centre for Microbiology, Madrid, Spain |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/36710969$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_jbc_2023_105198 |
| Cites_doi | 10.1128/AAC.00172-08 10.1038/nrmicro.2017.148 10.1038/nrmicro3525 10.1101/cshperspect.a025262 10.1186/2110-5820-1-14 10.1128/IAI.71.8.4250-4259.2003 10.1111/j.1574-6968.2011.02362.x 10.1111/j.1574-6976.2007.00094.x 10.1016/j.bioorg.2014.05.011 10.3389/fmed.2017.00163 10.1016/j.vaccine.2011.06.001 10.1111/j.1574-6968.2009.01669.x 10.1007/s00018-010-0571-8 10.3109/07388558909036741 10.1016/j.jhin.2011.08.013 10.1016/j.jiac.2016.01.010 10.1099/jmm.0.000636 10.1128/MMBR.00027-07 10.1186/s12866-015-0397-5 10.1007/s11274-018-2542-4 10.1111/j.1462-5822.2004.00443.x 10.1046/j.1365-2958.1999.01339.x 10.1128/JB.185.7.2374-2378.2003 10.1111/j.1574-6976.2012.00344.x 10.1128/AAC.01458-05 10.1074/jbc.M116.751099 10.1073/pnas.2003271117 10.1128/AAC.01314-12 10.1038/nmeth.2019 10.3389/fmicb.2019.00500 10.1186/s12866-020-02083-0 10.1128/microbiolspec.MTBP-0016-2017 10.3389/fcimb.2017.00055 10.1128/jb.179.8.2512-2518.1997 10.1128/AAC.00929-10 10.1111/j.1365-2818.2009.03299.x 10.1128/AEM.00280-20 10.1007/s40121-015-0092-8 10.1517/14656566.2014.914172 10.1038/nchembio.1289 10.1099/jmm.0.000874 10.1089/mdr.2016.0083 10.3390/biom8040122 10.1371/journal.pone.0017027 10.3389/fmicb.2015.00377 10.1093/cid/ciz830 10.1128/mBio.01783-16 10.1038/s41467-021-22849-y 10.1093/jac/dky289 10.3389/fpubh.2021.707435 10.1016/j.ijbiomac.2016.12.082 10.3389/fmicb.2019.00331 10.1111/j.1749-6632.2012.06813.x 10.1080/21505594.2018.1460187 10.1111/j.1365-2958.2005.04892.x 10.1016/j.femsre.2004.09.003 10.12998/wjcc.v2.i12.787 10.7717/peerj.9020 10.1046/j.1365-2958.1998.01062.x |
| ContentType | Journal Article |
| Copyright | Copyright © 2023 Tajuelo, Terrón, López-Siles and McConnell. Copyright © 2023 Tajuelo, Terrón, López-Siles and McConnell 2023 Tajuelo, Terrón, López-Siles and McConnell |
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| Keywords | biofilm formation Acinetobacter baumannii disinfectants fosfomycin resistance twitching motility peptidoglycan recycling |
| Language | English |
| License | Copyright © 2023 Tajuelo, Terrón, López-Siles and McConnell. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Paolo Visca, Roma Tre University, Italy The authors have contributed equally to this work and share senior authorship This article was submitted to Molecular Bacterial Pathogenesis, a section of the journal Frontiers in Cellular and Infection Microbiology Reviewed by: Chelsie Armbruster, University at Buffalo, United States; Alessandra Polissi, University of Milan, Italy |
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| References | Folkesson (B17) 2005; 7 Lin (B33) 2003; 71 Sastry (B49) 2016; 22 Schmidtke (B51) 2006; 50 Schwechheimer (B52) 2015; 13 Piepenbrink (B44) 2016; 291 Thanassi (B57) 1997; 179 Eijkelkamp (B14) 2011; 323 Gallant (B18) 2005; 58 Rumbo (B47) 2011; 55 Venter (B60) 2015; 6 Luo (B36) 2015; 15 Chiang (B9) 2003; 185 Lee (B32) 2017; 7 Doi (B12) 2019; 69 Shehadul Islam (B53) 2017 Silver (B54) 2017; 7 Candel (B6) 2019; 32 Dhar (B11) 2018; 67 Karaiskos (B29) 2014; 15 Rolain (B46) 2013; 57 Horinouchi (B22) 2018; 34 Johnson (B28) 2013; 1277 Kim (B30) 2021; 21 Carretero-Ledesma (B7) 2018; 9 Umerska (B58) 2018; 8 Wall (B63) 1999; 32 Lin (B35) 2020; 8 Cava (B8) 2011; 68 Espinal (B15) 2012; 80 Vermassen (B61) 2019; 10 San Millan (B48) 2017; 5 (B10) 2017 Urdaneta (B59) 2017; 4 Lin (B34) 2014; 2 Harding (B21) 2018; 16 Iarikov (B23) 2015; 4 Schindelin (B50) 2012; 9 Begley (B3) 2005; 29 Fisher (B16) 2014; 56 Bolla (B5) 2020; 117 Spapen (B56) 2011; 1 Moya (B39) 2008; 52 Irazoki (B25) 2019; 10 Jin (B27) 2011; 6 McConnell (B37) 2013; 37 Alonso-Del Valle (B2) 2021; 12 Park (B42) 2008; 72 Kwon (B31) 2009; 297 Aghamali (B1) 2019; 68 Gisin (B20) 2013; 9 Ingram (B24) 1989; 9 Bleck (B4) 2010; 237 Gil-Marqués (B19) 2018; 73 Rivera (B45) 2016; 22 Múñez Rubio (B40) 2019; 32 Pérez-Gallego (B43) 2016; 7 Sonkar (B55) 2017; 97 Vollmer (B62) 2008; 32 Domenech (B13) 2020; 86 O'Toole (B41) 1998; 30 Jiang (B26) 2021; 9 McConnell (B38) 2011; 29 |
| References_xml | – volume: 52 start-page: 1098 year: 2008 ident: B39 article-title: Benefit of having multiple ampD genes for acquiring beta-lactam resistance without losing fitness and virulence in Pseudomonas aeruginosa publication-title: Antimicrob Agent Chemother doi: 10.1128/AAC.00172-08 contributor: fullname: Moya – volume: 16 start-page: 91 year: 2018 ident: B21 article-title: Uncovering the mechanisms of Acinetobacter baumannii virulence publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro.2017.148 contributor: fullname: Harding – volume: 13 start-page: 605 year: 2015 ident: B52 article-title: Outer-membrane vesicles from gram-negative bacteria: Biogenesis and functions publication-title: Nat. Rev. Microbiol. doi: 10.1038/nrmicro3525 contributor: fullname: Schwechheimer – volume: 7 year: 2017 ident: B54 article-title: Fosfomycin: Mechanism and resistance publication-title: Cold Spring Harb. Perspect. Med. doi: 10.1101/cshperspect.a025262 contributor: fullname: Silver – volume: 1 start-page: 14 year: 2011 ident: B56 article-title: Renal and neurological side effects of colistin in critically ill patients publication-title: Ann. Intensive Care doi: 10.1186/2110-5820-1-14 contributor: fullname: Spapen – volume: 71 start-page: 4250 year: 2003 ident: B33 article-title: Critical role of multidrug efflux pump CmeABC in bile resistance and in vivo colonization of Campylobacter jejuni publication-title: Infect. Immun. doi: 10.1128/IAI.71.8.4250-4259.2003 contributor: fullname: Lin – volume: 323 start-page: 44 year: 2011 ident: B14 article-title: Adherence and motility characteristics of clinical Acinetobacter baumannii isolates publication-title: FEMS Microbiol. Lett. doi: 10.1111/j.1574-6968.2011.02362.x contributor: fullname: Eijkelkamp – volume: 32 start-page: 149 year: 2008 ident: B62 article-title: Peptidoglycan structure and architecture publication-title: FEMS Microbiol. Rev. doi: 10.1111/j.1574-6976.2007.00094.x contributor: fullname: Vollmer – volume-title: CLSI document M100-S27 year: 2017 ident: B10 article-title: Performance standards for antimicrobial susceptibility testing; twenty-seventh informational supplement – volume: 56 start-page: 41 year: 2014 ident: B16 article-title: The sentinel role of peptidoglycan recycling in the β-lactam resistance of the gram-negative enterobacteriaceae and Pseudomonas aeruginosa publication-title: Bioorg. Chem. doi: 10.1016/j.bioorg.2014.05.011 contributor: fullname: Fisher – volume: 4 year: 2017 ident: B59 article-title: Interactions between bacteria and bile salts in the gastrointestinal and hepatobiliary tracts publication-title: Front. Med. (Lausanne) doi: 10.3389/fmed.2017.00163 contributor: fullname: Urdaneta – volume: 29 start-page: 5705 year: 2011 ident: B38 article-title: Outer membrane vesicles as an acellular vaccine against Acinetobacter baumannii publication-title: Vaccine doi: 10.1016/j.vaccine.2011.06.001 contributor: fullname: McConnell – volume: 297 start-page: 150 year: 2009 ident: B31 article-title: Proteome analysis of outer membrane vesicles from a clinical Acinetobacter baumannii isolate publication-title: FEMS Microbiol. Lett. doi: 10.1111/j.1574-6968.2009.01669.x contributor: fullname: Kwon – volume: 68 start-page: 817 year: 2011 ident: B8 article-title: Emerging knowledge of regulatory roles of d-amino acids in bacteria publication-title: Cell. Mol. Life Sci. doi: 10.1007/s00018-010-0571-8 contributor: fullname: Cava – volume: 9 start-page: 305 year: 1989 ident: B24 article-title: Ethanol tolerance in bacteria publication-title: Crit. Rev. Biotechnol. doi: 10.3109/07388558909036741 contributor: fullname: Ingram – volume: 80 start-page: 56 year: 2012 ident: B15 article-title: Effect of biofilm formation on the survival of Acinetobacter baumannii on dry surfaces publication-title: J. Hosp. Infect. doi: 10.1016/j.jhin.2011.08.013 contributor: fullname: Espinal – volume: 22 start-page: 273 year: 2016 ident: B49 article-title: Fosfomycin: Resurgence of an old companion publication-title: J. Infect. Chemother. doi: 10.1016/j.jiac.2016.01.010 contributor: fullname: Sastry – volume: 67 start-page: 1 year: 2018 ident: B11 article-title: Cell-wall recycling and synthesis in Escherichia coli and Pseudomonas aeruginosa - their role in the development of resistance publication-title: J. Med. Microbiol. doi: 10.1099/jmm.0.000636 contributor: fullname: Dhar – volume: 72 start-page: 211 year: 2008 ident: B42 article-title: How bacteria consume their own exoskeletons (Turnover and recycling of cell wall peptidoglycan) publication-title: Microbiol. Mol. Biol. Rev. doi: 10.1128/MMBR.00027-07 contributor: fullname: Park – volume: 15 start-page: 62 year: 2015 ident: B36 article-title: Enhancing pili assembly and biofilm formation in Acinetobacter baumannii ATCC19606 using non-native acyl-homoserine lactones publication-title: BMC Microbiol. doi: 10.1186/s12866-015-0397-5 contributor: fullname: Luo – volume: 34 start-page: 157 year: 2018 ident: B22 article-title: Understanding and engineering alcohol-tolerant bacteria using OMICS technology publication-title: World J. Microbiol. Biotechnol. doi: 10.1007/s11274-018-2542-4 contributor: fullname: Horinouchi – volume: 7 start-page: 147 year: 2005 ident: B17 article-title: Components of the peptidoglycan-recycling pathway modulate invasion and intracellular survival of Salmonella enterica serovar Typhimurium publication-title: Cell Microbiol. doi: 10.1111/j.1462-5822.2004.00443.x contributor: fullname: Folkesson – volume: 32 start-page: 1 year: 1999 ident: B63 article-title: Type IV pili and cell motility publication-title: Mol. Microbiol. doi: 10.1046/j.1365-2958.1999.01339.x contributor: fullname: Wall – volume: 185 start-page: 2374 year: 2003 ident: B9 article-title: Biofilm formation by hyperpiliated mutants of Pseudomonas aeruginosa publication-title: J. Bacteriol. doi: 10.1128/JB.185.7.2374-2378.2003 contributor: fullname: Chiang – volume: 37 start-page: 130 year: 2013 ident: B37 article-title: Acinetobacter baumannii: human infections, factors contributing to pathogenesis and animal models publication-title: FEMS Microbiol. Rev. doi: 10.1111/j.1574-6976.2012.00344.x contributor: fullname: McConnell – volume: 50 start-page: 2030 year: 2006 ident: B51 article-title: Model system to evaluate the effect of ampD mutations on AmpC-mediated beta-lactam resistance publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.01458-05 contributor: fullname: Schmidtke – volume: 291 start-page: 22924 year: 2016 ident: B44 article-title: Structural diversity in the type IV pili of multidrug-resistant Acinetobacter publication-title: J. Biol. Chem. doi: 10.1074/jbc.M116.751099 contributor: fullname: Piepenbrink – volume: 117 start-page: 17011 year: 2020 ident: B5 article-title: Assembly and regulation of the chlorhexidine-specific efflux pump AceI publication-title: Proc. Natl. Acad. Sci. U.S.A. doi: 10.1073/pnas.2003271117 contributor: fullname: Bolla – volume: 57 start-page: 592 year: 2013 ident: B46 article-title: Real-time sequencing to decipher the molecular mechanism of resistance of a clinical pan-drug-resistant Acinetobacter baumannii isolate from Marseille, France publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.01314-12 contributor: fullname: Rolain – volume: 9 start-page: 676 year: 2012 ident: B50 article-title: Fiji: An open-source platform for biological-image analysis publication-title: Nat. Methods doi: 10.1038/nmeth.2019 contributor: fullname: Schindelin – volume: 10 year: 2019 ident: B25 article-title: Peptidoglycan muropeptides: Release, perception, and functions as signaling molecules publication-title: Front. Microbiol. doi: 10.3389/fmicb.2019.00500 contributor: fullname: Irazoki – volume: 21 start-page: 27 year: 2021 ident: B30 article-title: The role of zur-regulated lipoprotein a in bacterial morphology, antimicrobial susceptibility, and production of outer membrane vesicles in Acinetobacter baumannii publication-title: BMC Microbiol. doi: 10.1186/s12866-020-02083-0 contributor: fullname: Kim – volume: 32 start-page: 1 year: 2019 ident: B6 article-title: New perspectives for reassessing fosfomycin: Applicability in current clinical practice publication-title: Rev. Esp. Quimioter contributor: fullname: Candel – volume: 5 year: 2017 ident: B48 article-title: Fitness costs of plasmids: a limit to plasmid transmission publication-title: Microbiol. Spectr. doi: 10.1128/microbiolspec.MTBP-0016-2017 contributor: fullname: San Millan – volume: 7 year: 2017 ident: B32 article-title: Biology of Acinetobacter baumannii: Pathogenesis, antibiotic resistance mechanisms, and prospective treatment options publication-title: Front. Cell Infect. Microbiol. doi: 10.3389/fcimb.2017.00055 contributor: fullname: Lee – volume: 179 start-page: 2512 year: 1997 ident: B57 article-title: Active efflux of bile salts by Escherichia coli publication-title: J. Bacteriol. doi: 10.1128/jb.179.8.2512-2518.1997 contributor: fullname: Thanassi – volume: 55 start-page: 3084 year: 2011 ident: B47 article-title: Horizontal transfer of the OXA-24 carbapenemase gene via outer membrane vesicles: A new mechanism of dissemination of carbapenem resistance genes in Acinetobacter baumannii publication-title: Antimicrob. Agents Chemother. doi: 10.1128/AAC.00929-10 contributor: fullname: Rumbo – volume: 237 start-page: 23 year: 2010 ident: B4 article-title: Comparison of different methods for thin section EM analysis of mycobacterium smegmatis publication-title: J. Microsc. doi: 10.1111/j.1365-2818.2009.03299.x contributor: fullname: Bleck – volume: 86 year: 2020 ident: B13 article-title: The n-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in the structure and formation of biofilms publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.00280-20 contributor: fullname: Domenech – volume: 4 start-page: 433 year: 2015 ident: B23 article-title: Adverse events associated with fosfomycin use: Review of the literature and analyses of the FDA adverse event reporting system database publication-title: Infect. Dis. Ther. doi: 10.1007/s40121-015-0092-8 contributor: fullname: Iarikov – volume: 15 start-page: 1351 year: 2014 ident: B29 article-title: Multidrug-resistant and extensively drug-resistant gram-negative pathogens: Current and emerging therapeutic approaches publication-title: Expert Opin. Pharmacother. doi: 10.1517/14656566.2014.914172 contributor: fullname: Karaiskos – volume: 9 start-page: 491 year: 2013 ident: B20 article-title: A cell wall recycling shortcut that bypasses peptidoglycan de novo biosynthesis publication-title: Nat. Chem. Biol. doi: 10.1038/nchembio.1289 contributor: fullname: Gisin – volume: 68 start-page: 11 year: 2019 ident: B1 article-title: Fosfomycin: Mechanisms and the increasing prevalence of resistance publication-title: J. Med. Microbiol. doi: 10.1099/jmm.0.000874 contributor: fullname: Aghamali – volume: 22 start-page: 470 year: 2016 ident: B45 article-title: Orthologous and paralogous AmpD peptidoglycan amidases from gram-negative bacteria publication-title: Microb. Drug Resist. doi: 10.1089/mdr.2016.0083 contributor: fullname: Rivera – volume: 8 start-page: 122 year: 2018 ident: B58 article-title: Synergistic effect of combinations containing EDTA and the antimicrobial peptide AA230, an arenicin-3 derivative, on gram-negative bacteria publication-title: Biomolecules doi: 10.3390/biom8040122 contributor: fullname: Umerska – volume: 6 year: 2011 ident: B27 article-title: Acinetobacter baumannii secretes cytotoxic outer membrane protein a via outer membrane vesicles publication-title: PloS One doi: 10.1371/journal.pone.0017027 contributor: fullname: Jin – volume: 6 year: 2015 ident: B60 article-title: RND-type drug efflux pumps from gram-negative bacteria: Molecular mechanism and inhibition publication-title: Front. Microbiol. doi: 10.3389/fmicb.2015.00377 contributor: fullname: Venter – volume: 69 start-page: S565 year: 2019 ident: B12 article-title: Treatment options for carbapenem-resistant gram-negative bacterial infections publication-title: Clin. Infect. Dis. doi: 10.1093/cid/ciz830 contributor: fullname: Doi – volume: 7 year: 2016 ident: B43 article-title: Impact of AmpC derepression on fitness and virulence: The mechanism or the pathway publication-title: mBio doi: 10.1128/mBio.01783-16 contributor: fullname: Pérez-Gallego – volume: 12 start-page: 2653 year: 2021 ident: B2 article-title: Variability of plasmid fitness effects contributes to plasmid persistence in bacterial communities publication-title: Nat. Commun. doi: 10.1038/s41467-021-22849-y contributor: fullname: Alonso-Del Valle – volume: 73 start-page: 2960 year: 2018 ident: B19 article-title: Peptidoglycan recycling contributes to intrinsic resistance to fosfomycin in Acinetobacter baumannii publication-title: J. Antimicrob. Chemother. doi: 10.1093/jac/dky289 contributor: fullname: Gil-Marqués – volume: 9 year: 2021 ident: B26 article-title: Epidemiological analysis of multidrug-resistant Acinetobacter baumannii isolates in a tertiary hospital over a 12-year period in China publication-title: Front. Public Health doi: 10.3389/fpubh.2021.707435 contributor: fullname: Jiang – volume: 97 start-page: 106 year: 2017 ident: B55 article-title: UDP-N-Acetylglucosamine enolpyruvyl transferase (MurA) of Acinetobacter baumannii (AbMurA): Structural and functional properties publication-title: Int. J. Biol. Macromol. doi: 10.1016/j.ijbiomac.2016.12.082 contributor: fullname: Sonkar – volume: 10 year: 2019 ident: B61 article-title: Cell wall hydrolases in bacteria: Insight on the diversity of cell wall amidases, glycosidases and peptidases toward peptidoglycan publication-title: Front. Microbiol. doi: 10.3389/fmicb.2019.00331 contributor: fullname: Vermassen – volume: 1277 start-page: 54 year: 2013 ident: B28 article-title: Bacterial cell-wall recycling publication-title: Ann. N Y Acad. Sci. doi: 10.1111/j.1749-6632.2012.06813.x contributor: fullname: Johnson – volume: 9 start-page: 930 year: 2018 ident: B7 article-title: Phenotypic changes associated with colistin resistance due to lipopolysaccharide loss in Acinetobacter baumannii publication-title: Virulence doi: 10.1080/21505594.2018.1460187 contributor: fullname: Carretero-Ledesma – start-page: 83 volume-title: Micromachines (Basel) year: 2017 ident: B53 article-title: A review on macroscale and microscale cell lysis methods contributor: fullname: Shehadul Islam – volume: 58 start-page: 1012 year: 2005 ident: B18 article-title: Common beta-lactamases inhibit bacterial biofilm formation publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2005.04892.x contributor: fullname: Gallant – volume: 29 start-page: 625 year: 2005 ident: B3 article-title: The interaction between bacteria and bile publication-title: FEMS Microbiol. Rev. doi: 10.1016/j.femsre.2004.09.003 contributor: fullname: Begley – volume: 2 start-page: 787 year: 2014 ident: B34 article-title: Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside publication-title: World J. Clin. cases doi: 10.12998/wjcc.v2.i12.787 contributor: fullname: Lin – volume: 8 start-page: e9020 year: 2020 ident: B35 article-title: Characterization of biofilm production in different strains of Acinetobacter baumannii and the effects of chemical compounds on biofilm formation publication-title: PeerJ doi: 10.7717/peerj.9020 contributor: fullname: Lin – volume: 32 start-page: 62 year: 2019 ident: B40 article-title: Fosfomycin in antimicrobial stewardship programs publication-title: Rev. Esp. Quimioter contributor: fullname: Múñez Rubio – volume: 30 start-page: 295 year: 1998 ident: B41 article-title: Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development publication-title: Mol. Microbiol. doi: 10.1046/j.1365-2958.1998.01062.x contributor: fullname: O'Toole |
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| Title | Role of peptidoglycan recycling enzymes AmpD and AnmK in Acinetobacter baumannii virulence features |
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