Effect of Lactobacillus fermentum on Beta2 Toxin Production by Clostridium perfringens

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Published in:	Applied and Environmental Microbiology Vol. 77; no. 13; pp. 4406 - 4411
Main Authors:	ALLAART, Janneke G, VAN ASTERN, Alphons J. A. M, VERNOOIJ, Johannes C. M, GRÖNE, Andrea
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-07-2011
Subjects:	Animals Bacterial Toxins - antagonists & inhibitors Bacterial Toxins - biosynthesis Biological and medical sciences Carboxylic Acids - metabolism Clostridium perfringens - growth & development Clostridium perfringens - metabolism Digestive system Fundamental and applied biological sciences. Psychology Gastrointestinal diseases Gene Expression - drug effects Gene Expression Profiling Gram-positive bacteria Humans Hydrogen-Ion Concentration Lactobacillus fermentum - growth & development Microbial Ecology Microbial Interactions Microbial Viability Microbiology Toxins Toxin Lactic acid bacteria Lactobacillus fermentum Clostridiaceae Production Clostridiales Bacteria Lactobacillaceae Clostridium perfringens
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Abstract	Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: AEM
AbstractList	Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: AEM Clostridium perfringens, although a member of the normal gut flora, is also an important cause of intestinal disease in animals and, to a lesser extent, in humans. Disease is associated with the production of one or more toxins, and little is known about environmental influences on the production of these toxins. One of the health-promoting effects of lactic acid bacteria (LAB) is the establishment and maintenance of a low pH in the intestine since an acidic environment inhibits the growth of many potentially harmful bacteria. Here, the effect of the LAB Lactobacillus fermentum on beta2 toxin production by C. perfringens is described. Coculturing of C. perfringens with L. fermentum showed that under in vitro conditions, L. fermentum was capable of silencing beta2 toxin production by C. perfringens without influencing bacterial viability. The reduction in toxin production was shown to be most likely a result of the decline in pH. Quantitative PCR showed that the reduction in beta2 toxin production was due to a decrease in cpb2 mRNA. These results suggest that in the intestine, the production of beta2 toxin by C. perfringens might be regulated by other members of the normal intestinal flora. [PUBLICATION ABSTRACT] Clostridium perfringens , although a member of the normal gut flora, is also an important cause of intestinal disease in animals and, to a lesser extent, in humans. Disease is associated with the production of one or more toxins, and little is known about environmental influences on the production of these toxins. One of the health-promoting effects of lactic acid bacteria (LAB) is the establishment and maintenance of a low pH in the intestine since an acidic environment inhibits the growth of many potentially harmful bacteria. Here, the effect of the LAB Lactobacillus fermentum on beta2 toxin production by C. perfringens is described. Coculturing of C. perfringens with L. fermentum showed that under in vitro conditions, L. fermentum was capable of silencing beta2 toxin production by C. perfringens without influencing bacterial viability. The reduction in toxin production was shown to be most likely a result of the decline in pH. Quantitative PCR showed that the reduction in beta2 toxin production was due to a decrease in cpb2 mRNA. These results suggest that in the intestine, the production of beta2 toxin by C. perfringens might be regulated by other members of the normal intestinal flora. Clostridium perfringens, although a member of the normal gut flora, is also an important cause of intestinal disease in animals and, to a lesser extent, in humans. Disease is associated with the production of one or more toxins, and little is known about environmental influences on the production of these toxins. One of the health-promoting effects of lactic acid bacteria (LAB) is the establishment and maintenance of a low pH in the intestine since an acidic environment inhibits the growth of many potentially harmful bacteria. Here, the effect of the LAB Lactobacillus fermentum on beta2 toxin production by C. perfringens is described. Coculturing of C. perfringens with L. fermentum showed that under in vitro conditions, L. fermentum was capable of silencing beta2 toxin production by C. perfringens without influencing bacterial viability. The reduction in toxin production was shown to be most likely a result of the decline in pH. Quantitative PCR showed that the reduction in beta2 toxin production was due to a decrease in cpb2 mRNA. These results suggest that in the intestine, the production of beta2 toxin by C. perfringens might be regulated by other members of the normal intestinal flora.
Author	Johannes C. M. Vernooij Janneke G. Allaart Andrea Gröne Alphons J. A. M. van Asten
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Keywords	Toxin Lactic acid bacteria Lactobacillus fermentum Clostridiaceae Production Clostridiales Bacteria Lactobacillaceae Clostridium perfringens
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Snippet	Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit... Clostridium perfringens, although a member of the normal gut flora, is also an important cause of intestinal disease in animals and, to a lesser extent, in... Clostridium perfringens , although a member of the normal gut flora, is also an important cause of intestinal disease in animals and, to a lesser extent, in...
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SubjectTerms	Animals Bacterial Toxins - antagonists & inhibitors Bacterial Toxins - biosynthesis Biological and medical sciences Carboxylic Acids - metabolism Clostridium perfringens - growth & development Clostridium perfringens - metabolism Digestive system Fundamental and applied biological sciences. Psychology Gastrointestinal diseases Gene Expression - drug effects Gene Expression Profiling Gram-positive bacteria Humans Hydrogen-Ion Concentration Lactobacillus fermentum - growth & development Microbial Ecology Microbial Interactions Microbial Viability Microbiology Toxins
Title	Effect of Lactobacillus fermentum on Beta2 Toxin Production by Clostridium perfringens
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