Induction of Protective Immunity against Schistosoma mansoni by Vaccination with Schistosome Paramyosin (Sm97), a Nonsurface Parasite Antigen

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 85; no. 15; pp. 5678 - 5682
• Main Authors: Pearce, E. J., James, S. L., Hieny, S., Lanar, D. E., Sher, A.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01-08-1988; National Acad Sciences
• Subjects: Animals; Antigens; Antigens, Helminth - immunology; Biological and medical sciences; Diseases caused by trematodes; Epitopes; Female; Helminthic diseases; Immunity, Cellular; Immunization; Immunization, Passive; Infections; Infectious diseases; Injections, Intradermal; Interferon-gamma - biosynthesis; Macrophages - immunology; Medical sciences; Mice; Molecules; Mycobacterium bovis - immunology; Parasites; Parasitic diseases; Schistosoma mansoni; Schistosoma mansoni - immunology; Schistosomiases; Schistosomiasis mansoni - prevention & control; Schistosomula; T lymphocytes; T-Lymphocytes - immunology; Tropical medicine; Tropomyosin - immunology; Vaccination; Vaccines; Vaccines, Synthetic; Immunoprotection; Schistosoma mansoni; Induction; Rodentia; BCG; Plathelmintha; Vaccine; Experimental animal; Trematoda; Antigen; Proteins; Immunoprophylaxis; Vertebrata; Mammalia; Mouse; Helmintha; Invertebrata
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.85.15.5678

Paramyosin (Sm97), a 97-kDa myofibrillar protein identified by the unusually monospecific antibody response induced by intradermal vaccination of mice with a complex soluble worm antigen preparation (SWAP) of adult Schistosoma mansoni administered with bacillus Calmette-Guerin (BCG), was purified and tested for its capacity to protect mice against challenge infection. When administered intradermally with BCG at total doses of only 4-40 μ g per mouse, both the native molecule and a recombinant expression product containing approximately 50% of the whole protein were found to confer significant resistance (26-33%) against challenge infection, while 2 mg of unfractionated SWAP was required to induce similar levels of protection. In addition, paramyosin was shown to stimulate T lymphocytes from vaccinated mice to produce lymphokines [e.g., γ interferon (IFN-γ )] that activate macrophages to kill schistosomula. Neither schistosome myosin nor a heterologous paramyosin from a different invertebrate genus were protective, indicating a requirement for specific epitopes in the immunization. That the protection induced by paramyosin involves a T-cell-mediated mechanism was supported by the failure of antiparamyosin antibodies to passively transfer significant resistance to infection to recipient mice. Lymphocytes from mice vaccinated with paramyosin were found to produce IFN-γ in response to living schistosomula, suggesting that during challenge infection of vaccinated hosts, paramyosin (a nonsurface antigen) may elicit a protective T-cell response as a consequence of its release from migrating parasite larvae. Paramyosin-depleted SWAP was also found to be protective as well as stimulatory for T lymphocytes from SWAP-vaccinated mice, indicating that other antigens in this preparation may have immunoprophylactic potential. In summary, these results (i) suggest that the induction of T-cell-dependent cell-mediated immunity against soluble nonsurface antigens may be an effective strategy for immunization against multicellular parasites and (ii) in the case of schistosomes, identify paramyosin as a candidate vaccine immunogen in this category.