A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants
The SARS-CoV-2 P.1 variant, responsible for an outbreak in Manaus, Brazil, is distinguished by 12 amino acid differences in the S protein, potentially increasing its ACE-2 affinity and immune evasion capability. We investigated the innate immune response of this variant compared to the original B.1...
Saved in:
| Published in: | Microorganisms (Basel) Vol. 12; no. 4; p. 720 |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
MDPI AG
01-04-2024
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | The SARS-CoV-2 P.1 variant, responsible for an outbreak in Manaus, Brazil, is distinguished by 12 amino acid differences in the S protein, potentially increasing its ACE-2 affinity and immune evasion capability. We investigated the innate immune response of this variant compared to the original B.1 strain, particularly concerning cytokine production. Blood samples from three severe COVID-19 patients were analyzed post-infection with both strains. Results showed no significant difference in cytokine production of mononuclear cells and neutrophils for either variant. While B.1 had higher cytopathogenicity, neither showed viral replication in mononuclear cells. Structural analyses of the S protein highlighted physicochemical variations, which might be linked to the differences in infectivity between the strains. Our studies point to the increased infectivity of P.1 could stem from altered immunogenicity and receptor-binding affinity. |
|---|---|
| AbstractList | The SARS-CoV-2 P.1 variant, responsible for an outbreak in Manaus, Brazil, is distinguished by 12 amino acid differences in the S protein, potentially increasing its ACE-2 affinity and immune evasion capability. We investigated the innate immune response of this variant compared to the original B.1 strain, particularly concerning cytokine production. Blood samples from three severe COVID-19 patients were analyzed post-infection with both strains. Results showed no significant difference in cytokine production of mononuclear cells and neutrophils for either variant. While B.1 had higher cytopathogenicity, neither showed viral replication in mononuclear cells. Structural analyses of the S protein highlighted physicochemical variations, which might be linked to the differences in infectivity between the strains. Our studies point to the increased infectivity of P.1 could stem from altered immunogenicity and receptor-binding affinity. |
| Audience | Academic |
| Author | de Senna Silveira, Etiele Siqueira, Marilda Dahab, Elizabeth Chen Makhoul, Gustavo José Freire-de-Lima, Celio Geraldo Pauvolid-Corrêa, Alex Barroso, Shana Priscila Coutinho Valente, Natalia Fonseca, Leonardo Marques da Araújo, Mia Morrot, Alexandre Vieira, Gustavo Fioravanti Freire-de-Lima, Leonardo Nacife, Valeria da Silva-Junior, Elias Barbosa Nico, Dirlei Machado, Ana Diniz-Lima, Israel Scovino, Aline Miranda Cardoso, Karina Martins |
| Author_xml | – sequence: 1 givenname: Aline Miranda surname: Scovino fullname: Scovino, Aline Miranda organization: Laboratório de Imunoparasitologia, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil – sequence: 2 givenname: Elizabeth Chen orcidid: 0000-0003-3960-3434 surname: Dahab fullname: Dahab, Elizabeth Chen organization: Laboratório de Imunoparasitologia, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil – sequence: 3 givenname: Israel orcidid: 0000-0003-0176-3520 surname: Diniz-Lima fullname: Diniz-Lima, Israel organization: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil – sequence: 4 givenname: Etiele orcidid: 0000-0001-8133-8457 surname: de Senna Silveira fullname: de Senna Silveira, Etiele organization: Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91501-970, Brazil – sequence: 5 givenname: Shana Priscila Coutinho orcidid: 0000-0001-7890-9255 surname: Barroso fullname: Barroso, Shana Priscila Coutinho organization: Biomanguinhos, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Brazil – sequence: 6 givenname: Karina Martins orcidid: 0000-0002-0986-4193 surname: Cardoso fullname: Cardoso, Karina Martins organization: Laboratório de Biologia Molecular, Instituto de Pesquisa Biomédica, Hospital Naval Marcílio Dias, Marinha do Brazil, Rio de Janeiro 20725-090, Brazil – sequence: 7 givenname: Dirlei surname: Nico fullname: Nico, Dirlei organization: Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil – sequence: 8 givenname: Gustavo José surname: Makhoul fullname: Makhoul, Gustavo José organization: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil – sequence: 9 givenname: Elias Barbosa surname: da Silva-Junior fullname: da Silva-Junior, Elias Barbosa organization: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil – sequence: 10 givenname: Celio Geraldo orcidid: 0000-0002-4148-3657 surname: Freire-de-Lima fullname: Freire-de-Lima, Celio Geraldo organization: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil – sequence: 11 givenname: Leonardo orcidid: 0000-0003-3013-3173 surname: Freire-de-Lima fullname: Freire-de-Lima, Leonardo organization: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil – sequence: 12 givenname: Leonardo Marques da orcidid: 0000-0001-8239-6843 surname: Fonseca fullname: Fonseca, Leonardo Marques da organization: Curso de Medicina, Universidade Castelo Branco (UCB), Rio de Janeiro 21710-255, Brazil – sequence: 13 givenname: Natalia surname: Valente fullname: Valente, Natalia organization: Laboratório de Vírus Respiratórios e Sarampo, COVID-19 National Reference Laboratory of Brazil and World Health Organization COVID-19 Reference Laboratory, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil – sequence: 14 givenname: Valeria surname: Nacife fullname: Nacife, Valeria organization: Laboratório de Vírus Respiratórios e Sarampo, COVID-19 National Reference Laboratory of Brazil and World Health Organization COVID-19 Reference Laboratory, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil – sequence: 15 givenname: Ana orcidid: 0000-0002-0581-5415 surname: Machado fullname: Machado, Ana organization: Laboratório de Vírus Respiratórios e Sarampo, COVID-19 National Reference Laboratory of Brazil and World Health Organization COVID-19 Reference Laboratory, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil – sequence: 16 givenname: Mia surname: Araújo fullname: Araújo, Mia organization: Laboratório de Vírus Respiratórios e Sarampo, COVID-19 National Reference Laboratory of Brazil and World Health Organization COVID-19 Reference Laboratory, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil – sequence: 17 givenname: Gustavo Fioravanti orcidid: 0000-0002-2380-8059 surname: Vieira fullname: Vieira, Gustavo Fioravanti organization: PPGSDH-Programa de Pós-Graduação em Saúde e Desenvolvimento Humano, Universidade La Salle, Canoas 92010-000, Brazil – sequence: 18 givenname: Alex orcidid: 0000-0002-6924-157X surname: Pauvolid-Corrêa fullname: Pauvolid-Corrêa, Alex organization: Laboratório de Virologia Veterinária de Viçosa, Departamento de Veterinária, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil – sequence: 19 givenname: Marilda surname: Siqueira fullname: Siqueira, Marilda organization: Laboratório de Vírus Respiratórios e Sarampo, COVID-19 National Reference Laboratory of Brazil and World Health Organization COVID-19 Reference Laboratory, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-360, Brazil – sequence: 20 givenname: Alexandre surname: Morrot fullname: Morrot, Alexandre organization: Escola de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, Brazil |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38674664$$D View this record in MEDLINE/PubMed |
| BookMark | eNptks1u3CAUha0qVZOmeYUIqZtunGLzZy9HozYdKVKlTJutdcGQMDUwBRwpfYe-c5nMNP1RYAFcnfMBV-d1deSD11V13uALQnr83lkVQ4i34G1yqWkxxaLFL6qTFgtetxyLo7_2x9VZShtcRt-QjjWvqmPScUE5pyfVzwVaBreFCNnea7TwMD0km1AwaOU9ZI1Wzs1eo2udtsEnnRD4EeU7jdY5zirPESa0vCsAlXW0Pwon-J19vbXfNDIxOLReXK_rZbipW3QJzgG6gWjB5z1rPcv7w_lN9dLAlPTZYT2tvn788GX5qb76fLlaLq5qRbnItdAYg-JUyJFRqlosOWagRoPFSIVhggnOhGxbaHrFqG67XopRNmbUopgoOa1We-4YYDNso3UQH4YAdngslNYOELNVkx4okV3fAOsM1pRyIyWUzkpBjFGkafvCerdnbWP4PuuUB2eT0tMEXoc5DQRT0bOm60WRvv1PuglzLC1_VPG-p5yyP6pbKPdbb0Iuzd1Bh4XoCWOdoKSoLp5RlTnqEo-SF2NL_R8D3xtKdlKK2jz9u8HDLlbD87EqxvPDq2fp9Phk-x0i8gtKsM0j |
| Cites_doi | 10.1016/j.jmii.2020.03.022 10.1056/NEJMra2026131 10.1038/s41586-021-03944-y 10.1038/s41586-021-03777-9 10.3390/vaccines9111354 10.2807/1560-7917.ES.2021.26.16.2100348 10.1038/s41579-020-00459-7 10.1126/science.abd5223 10.1016/j.cell.2021.02.037 10.3389/fimmu.2021.647824 10.1038/s12276-021-00592-0 10.3390/vaccines9101195 10.1038/s41564-020-0695-z 10.7326/M22-0308 10.1101/2021.01.24.427939 10.1038/s41467-021-24360-w 10.1038/s41586-021-03426-1 10.1002/jmv.28875 10.1093/infdis/jiab044 10.1126/science.abh2644 10.1073/pnas.2106535118 10.1038/s41577-020-0328-z 10.3390/pathogens10060633 10.3389/fmed.2021.792135 10.1002/jmv.27613 10.15585/mmwr.mm7106e2 10.1007/s13167-021-00267-w 10.1136/bmj.n579 10.1016/j.jiac.2022.01.009 10.3390/v13030439 10.3201/eid2704.210138 10.2139/ssrn.3866560 10.1126/science.abf6950 10.1038/s41591-021-01318-5 10.1038/s41586-021-03470-x 10.1016/j.cell.2020.02.052 10.1016/j.celrep.2022.110829 10.1016/j.cyto.2020.155365 10.1016/j.celrep.2020.108175 10.3389/fmed.2022.912367 10.3346/jkms.2022.37.e31 10.1016/j.immuni.2021.06.003 10.1038/s41598-020-76781-0 10.1016/j.cell.2020.08.012 10.1016/S0140-6736(22)00017-4 10.46234/ccdcw2021.255 10.1093/cid/ciab721 10.37201/req/071.2021 10.1016/j.cell.2020.08.001 10.1016/S0140-6736(20)30251-8 10.1057/s41271-021-00302-0 10.1038/s41392-020-00369-3 10.1038/s41586-021-04266-9 10.3390/v13112178 10.3389/fpubh.2021.784300 10.1001/jama.2020.1585 10.1503/cmaj.211248 10.1001/jama.2021.24868 10.1016/j.immuni.2022.06.004 10.1016/j.xcrm.2020.100166 10.1038/s41467-020-17665-9 10.3389/fimmu.2021.668507 10.1164/rccm.201410-1782OC |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2024 MDPI AG 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: COPYRIGHT 2024 MDPI AG – notice: 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | NPM AAYXX CITATION 7T7 8FD 8FE 8FH ABUWG AFKRA ATCPS AZQEC BBNVY BENPR BHPHI C1K CCPQU DWQXO FR3 GNUQQ HCIFZ LK8 M7P P64 PATMY PIMPY PQEST PQQKQ PQUKI PYCSY 7X8 DOA |
| DOI | 10.3390/microorganisms12040720 |
| DatabaseName | PubMed CrossRef Industrial and Applied Microbiology Abstracts (Microbiology A) Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Collection ProQuest Central (Alumni) ProQuest Central ProQuest Agriculture & Environmental Science Database ProQuest Central Essentials Biological Science Collection ProQuest Central ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Central Engineering Research Database ProQuest Central Student SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Biological Science Collection Biological Science Database Biotechnology and BioEngineering Abstracts Environmental Science Database Publicly Available Content Database (Proquest) (PQ_SDU_P3) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition Environmental Science Collection MEDLINE - Academic DOAJ Directory of Open Access Journals |
| DatabaseTitle | PubMed CrossRef Publicly Available Content Database ProQuest Central Student Technology Research Database ProQuest Central Essentials ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection Environmental Sciences and Pollution Management ProQuest Central Natural Science Collection ProQuest Central Korea Agricultural & Environmental Science Collection Biological Science Collection Industrial and Applied Microbiology Abstracts (Microbiology A) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Biological Science Database ProQuest SciTech Collection Biotechnology and BioEngineering Abstracts Environmental Science Collection ProQuest One Academic UKI Edition Environmental Science Database Engineering Research Database ProQuest One Academic MEDLINE - Academic |
| DatabaseTitleList | PubMed CrossRef MEDLINE - Academic Publicly Available Content Database |
| Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: http://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 2076-2607 |
| ExternalDocumentID | oai_doaj_org_article_43b891a58f0e446fbba207b73ffc3129 A793558743 10_3390_microorganisms12040720 38674664 |
| Genre | Journal Article |
| GeographicLocations | Brazil |
| GeographicLocations_xml | – name: Brazil |
| GroupedDBID | 53G 5VS 7XC 8FE 8FH AADQD AAFWJ AAHBH ACPRK AFKRA AFPKN AFRAH AFZYC ALMA_UNASSIGNED_HOLDINGS ATCPS BBNVY BENPR BHPHI CCPQU ECGQY GROUPED_DOAJ GS5 GX1 HCIFZ HYE IAO ITC KQ8 LK8 M48 M7P MODMG M~E NPM OK1 PATMY PGMZT PIMPY PROAC PYCSY RIG RNS RPM AAYXX CITATION 7T7 8FD ABUWG AZQEC C1K DWQXO FR3 GNUQQ P64 PQEST PQQKQ PQUKI 7X8 |
| ID | FETCH-LOGICAL-c467t-7e00ac647bd544c20b605acdf07d47f5757657b22a19c54e289b7db1fde747b43 |
| IEDL.DBID | DOA |
| ISSN | 2076-2607 |
| IngestDate | Tue Oct 22 15:12:48 EDT 2024 Sat Oct 26 05:17:49 EDT 2024 Mon Nov 04 15:37:19 EST 2024 Tue Nov 19 21:31:02 EST 2024 Tue Nov 12 23:36:34 EST 2024 Thu Nov 21 23:51:24 EST 2024 Sat Nov 02 12:31:17 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | COVID-19 mononuclear cells B.1 strain SARS-CoV-2 cytokines neutrophils P.1 variant |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c467t-7e00ac647bd544c20b605acdf07d47f5757657b22a19c54e289b7db1fde747b43 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0003-0176-3520 0000-0003-3960-3434 0000-0001-7890-9255 0000-0002-2380-8059 0000-0001-8133-8457 0000-0003-3013-3173 0000-0002-0581-5415 0000-0002-4148-3657 0000-0002-0986-4193 0000-0001-8239-6843 0000-0002-6924-157X |
| OpenAccessLink | https://doaj.org/article/43b891a58f0e446fbba207b73ffc3129 |
| PMID | 38674664 |
| PQID | 3046994645 |
| PQPubID | 2032358 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_43b891a58f0e446fbba207b73ffc3129 proquest_miscellaneous_3047951897 proquest_journals_3046994645 gale_infotracmisc_A793558743 gale_infotracacademiconefile_A793558743 crossref_primary_10_3390_microorganisms12040720 pubmed_primary_38674664 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-04-01 |
| PublicationDateYYYYMMDD | 2024-04-01 |
| PublicationDate_xml | – month: 04 year: 2024 text: 2024-04-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland – name: Basel |
| PublicationTitle | Microorganisms (Basel) |
| PublicationTitleAlternate | Microorganisms |
| PublicationYear | 2024 |
| Publisher | MDPI AG |
| Publisher_xml | – name: MDPI AG |
| References | Tragni (ref_10) 2022; 13 Liu (ref_4) 2021; 138 Houhamdi (ref_52) 2022; 94 Levi (ref_26) 2021; 9 Khare (ref_58) 2021; 3 Niles (ref_61) 2021; 12 Saito (ref_33) 2022; 602 Lu (ref_7) 2020; 395 Planas (ref_30) 2021; 596 ref_59 Liu (ref_16) 2020; 81 Pacha (ref_5) 2020; 20 Davies (ref_39) 2021; 593 Pierri (ref_22) 2020; 5 Mousavizadeh (ref_8) 2021; 54 Boumaza (ref_63) 2021; 224 Zhou (ref_36) 2021; 184 Wang (ref_31) 2021; 54 ref_25 ref_24 Hu (ref_12) 2021; 19 ref_21 ref_20 Zizza (ref_6) 2021; 62 ref_62 Singh (ref_11) 2020; 32 Starr (ref_19) 2020; 182 Burnett (ref_66) 2022; 55 Kim (ref_53) 2022; 37 Hu (ref_43) 2021; 8 Imai (ref_55) 2021; 118 Challen (ref_38) 2021; 372 Muir (ref_17) 2016; 193 Bouzid (ref_48) 2022; 175 Mlcochova (ref_34) 2021; 599 Wolter (ref_50) 2022; 399 Ong (ref_40) 2021; 75 (ref_47) 2021; 34 Reusch (ref_65) 2020; 182 Kim (ref_13) 2021; 53 Lei (ref_14) 2020; 11 Fisman (ref_45) 2021; 193 Modes (ref_51) 2022; 71 Fajgenbaum (ref_15) 2020; 383 Funk (ref_44) 2021; 26 Coperchini (ref_57) 2021; 12 Volz (ref_18) 2021; 593 Wang (ref_3) 2020; 323 Planas (ref_37) 2021; 27 Shoji (ref_42) 2022; 28 ref_46 Hoffmann (ref_9) 2020; 181 Faria (ref_27) 2021; 372 Liu (ref_32) 2022; 39 ref_41 Chevrier (ref_64) 2021; 2 ref_1 ref_2 Sikora (ref_23) 2020; 370 Arcanjo (ref_56) 2020; 10 Vanderbeke (ref_60) 2021; 12 Maslo (ref_49) 2022; 327 Zhang (ref_54) 2022; 9 McCarthy (ref_29) 2021; 371 Tragni (ref_35) 2023; 95 Ferrante (ref_67) 2021; 42 Fujino (ref_28) 2021; 27 |
| References_xml | – volume: 54 start-page: 159 year: 2021 ident: ref_8 article-title: Genotype and phenotype of COVID-19: Their roles in pathogenesis publication-title: J. Microbiol. Immunol. Infect. doi: 10.1016/j.jmii.2020.03.022 contributor: fullname: Mousavizadeh – volume: 383 start-page: 2255 year: 2020 ident: ref_15 article-title: Cytokine Storm publication-title: N. Engl. J. Med. doi: 10.1056/NEJMra2026131 contributor: fullname: Fajgenbaum – volume: 599 start-page: 114 year: 2021 ident: ref_34 article-title: SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion publication-title: Nature doi: 10.1038/s41586-021-03944-y contributor: fullname: Mlcochova – volume: 596 start-page: 276 year: 2021 ident: ref_30 article-title: Reduced sensitivity of SARS-CoV-2 variant Delta to antibody neutralization publication-title: Nature doi: 10.1038/s41586-021-03777-9 contributor: fullname: Planas – ident: ref_41 doi: 10.3390/vaccines9111354 – volume: 26 start-page: 2100348 year: 2021 ident: ref_44 article-title: Characteristics of SARS-CoV-2 variants of concern B.1.1.7, B.1.351 or P.1: Data from seven EU/EEA countries, weeks 38/2020 to 10/2021 publication-title: Eurosurveillance doi: 10.2807/1560-7917.ES.2021.26.16.2100348 contributor: fullname: Funk – volume: 19 start-page: 141 year: 2021 ident: ref_12 article-title: Characteristics of SARS-CoV-2 and COVID-19 publication-title: Nat. Rev. Microbiol. doi: 10.1038/s41579-020-00459-7 contributor: fullname: Hu – volume: 370 start-page: 203 year: 2020 ident: ref_23 article-title: In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges publication-title: Science doi: 10.1126/science.abd5223 contributor: fullname: Sikora – volume: 184 start-page: 2348 year: 2021 ident: ref_36 article-title: Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine-induced sera publication-title: Cell doi: 10.1016/j.cell.2021.02.037 contributor: fullname: Zhou – volume: 12 start-page: 647824 year: 2021 ident: ref_61 article-title: Macrophages and Dendritic Cells Are Not the Major Source of Pro-Inflammatory Cytokines Upon SARS-CoV-2 Infection publication-title: Front. Immunol. doi: 10.3389/fimmu.2021.647824 contributor: fullname: Niles – volume: 53 start-page: 750 year: 2021 ident: ref_13 article-title: Type I and III interferon responses in SARS-CoV-2 infection publication-title: Exp. Mol. Med. doi: 10.1038/s12276-021-00592-0 contributor: fullname: Kim – ident: ref_25 doi: 10.3390/vaccines9101195 – ident: ref_1 doi: 10.1038/s41564-020-0695-z – volume: 175 start-page: 831 year: 2022 ident: ref_48 article-title: Comparison of Patients Infected with Delta Versus Omicron COVID-19 Variants Presenting to Paris Emergency Departments: A Retrospective Cohort Study publication-title: Ann. Intern. Med. doi: 10.7326/M22-0308 contributor: fullname: Bouzid – ident: ref_20 doi: 10.1101/2021.01.24.427939 – volume: 12 start-page: 4117 year: 2021 ident: ref_60 article-title: Monocyte-driven atypical cytokine storm and aberrant neutrophil activation as key mediators of COVID-19 disease severity publication-title: Nat. Commun. doi: 10.1038/s41467-021-24360-w contributor: fullname: Vanderbeke – volume: 593 start-page: 270 year: 2021 ident: ref_39 article-title: Increased mortality in community-tested cases of SARS-CoV-2 lineage B.1.1.7 publication-title: Nature doi: 10.1038/s41586-021-03426-1 contributor: fullname: Davies – volume: 95 start-page: e28875 year: 2023 ident: ref_35 article-title: Deconstructing SARS-CoV-2 Neutralization: A Modular Molecular Framework for Computational Design and Comparison of Antibodies and Nanobodies Targeting the Spike RBD publication-title: J. Med. Virol. doi: 10.1002/jmv.28875 contributor: fullname: Tragni – volume: 224 start-page: 395 year: 2021 ident: ref_63 article-title: Monocytes and Macrophages, Targets of Severe Acute Respiratory Syndrome Coronavirus 2: The Clue for Coronavirus Disease 2019 Immunoparalysis publication-title: J. Infect. Dis. doi: 10.1093/infdis/jiab044 contributor: fullname: Boumaza – volume: 372 start-page: 815 year: 2021 ident: ref_27 article-title: Genomics and epidemiology of the P.1 SARS-CoV-2 lineage in Manaus, Brazil publication-title: Science doi: 10.1126/science.abh2644 contributor: fullname: Faria – volume: 118 start-page: e2106535118 year: 2021 ident: ref_55 article-title: Characterization of a new SARS-CoV-2 variant that emerged in Brazil publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.2106535118 contributor: fullname: Imai – volume: 20 start-page: 345 year: 2020 ident: ref_5 article-title: COVID-19: A case for inhibiting IL-17? publication-title: Nat. Rev. Immunol. doi: 10.1038/s41577-020-0328-z contributor: fullname: Pacha – ident: ref_21 doi: 10.3390/pathogens10060633 – volume: 8 start-page: 792135 year: 2021 ident: ref_43 article-title: Differences in Clinical Characteristics Between Delta Variant and Wild-Type SARS-CoV-2 Infected Patients publication-title: Front. Med. doi: 10.3389/fmed.2021.792135 contributor: fullname: Hu – volume: 94 start-page: 2290 year: 2022 ident: ref_52 article-title: Characteristics of the first 1119 SARS-CoV-2 Omicron variant cases, in Marseille, France, November–December 2021 publication-title: J. Med. Virol. doi: 10.1002/jmv.27613 contributor: fullname: Houhamdi – volume: 71 start-page: 217 year: 2022 ident: ref_51 article-title: Clinical Characteristics and Outcomes among Adults Hospitalized with Laboratory-Confirmed SARS-CoV-2 Infection during Periods of B.1.617.2 (Delta) and B.1.1.529 (Omicron) Variant Predominance—One Hospital, California, July 15-September 23, 2021, and December 21, 2021–January 27, 2022 publication-title: MMWR Morb. Mortal. Wkly. Rep. doi: 10.15585/mmwr.mm7106e2 contributor: fullname: Modes – volume: 13 start-page: 149 year: 2022 ident: ref_10 article-title: Modeling SARS-CoV-2 spike/ACE2 protein–protein interactions for predicting the binding affinity of new spike variants for ACE2, and novel ACE2 structurally related human protein targets, for COVID-19 handling in the 3PM context publication-title: EPMA J. doi: 10.1007/s13167-021-00267-w contributor: fullname: Tragni – volume: 372 start-page: n579 year: 2021 ident: ref_38 article-title: Risk of mortality in patients infected with SARS-CoV-2 variant of concern 202012/1: Matched cohort study publication-title: BMJ doi: 10.1136/bmj.n579 contributor: fullname: Challen – volume: 28 start-page: 591 year: 2022 ident: ref_42 article-title: Comparison of the clinical characteristics and outcomes of COVID-19 in children before and after the emergence of Delta variant of concern in Japan publication-title: J. Infect. Chemother. doi: 10.1016/j.jiac.2022.01.009 contributor: fullname: Shoji – ident: ref_24 doi: 10.3390/v13030439 – ident: ref_59 – volume: 27 start-page: 1243 year: 2021 ident: ref_28 article-title: Novel SARS-CoV-2 Variant in Travelers from Brazil to Japan publication-title: Emerg. Infect. Dis. doi: 10.3201/eid2704.210138 contributor: fullname: Fujino – ident: ref_46 doi: 10.2139/ssrn.3866560 – volume: 62 start-page: E13 year: 2021 ident: ref_6 article-title: Clinical features of COVID-19 and SARS epidemics. A literature review publication-title: J. Prev. Med. Hyg. contributor: fullname: Zizza – volume: 371 start-page: 1139 year: 2021 ident: ref_29 article-title: Recurrent deletions in the SARS-CoV-2 spike glycoprotein drive antibody escape publication-title: Science doi: 10.1126/science.abf6950 contributor: fullname: McCarthy – volume: 27 start-page: 917 year: 2021 ident: ref_37 article-title: Sensitivity of infectious SARS-CoV-2 B.1.1.7 and B.1.351 variants to neutralizing antibodies publication-title: Nat. Med. doi: 10.1038/s41591-021-01318-5 contributor: fullname: Planas – volume: 593 start-page: 266 year: 2021 ident: ref_18 article-title: Assessing transmissibility of SARS-CoV-2 lineage B.1.1.7 in England publication-title: Nature doi: 10.1038/s41586-021-03470-x contributor: fullname: Volz – volume: 181 start-page: 271 year: 2020 ident: ref_9 article-title: SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor publication-title: Cell doi: 10.1016/j.cell.2020.02.052 contributor: fullname: Hoffmann – volume: 39 start-page: 110829 year: 2022 ident: ref_32 article-title: Delta spike P681R mutation enhances SARS-CoV-2 fitness over Alpha variant publication-title: Cell Rep. doi: 10.1016/j.celrep.2022.110829 contributor: fullname: Liu – volume: 138 start-page: 155365 year: 2021 ident: ref_4 article-title: An inter-correlated cytokine network identified at the center of cytokine storm predicted COVID-19 prognosis publication-title: Cytokine doi: 10.1016/j.cyto.2020.155365 contributor: fullname: Liu – volume: 32 start-page: 108175 year: 2020 ident: ref_11 article-title: A Single-Cell RNA Expression Map of Human Coronavirus Entry Factors publication-title: Cell Rep. doi: 10.1016/j.celrep.2020.108175 contributor: fullname: Singh – volume: 9 start-page: 912367 year: 2022 ident: ref_54 article-title: Clinical Characteristics of COVID-19 Patients Infected by the Omicron Variant of SARS-CoV-2 publication-title: Front. Med. doi: 10.3389/fmed.2022.912367 contributor: fullname: Zhang – volume: 37 start-page: e31 year: 2022 ident: ref_53 article-title: Clinical Characteristics of 40 Patients Infected with the SARS-CoV-2 Omicron Variant in Korea publication-title: J. Korean Med. Sci. doi: 10.3346/jkms.2022.37.e31 contributor: fullname: Kim – volume: 54 start-page: 1611 year: 2021 ident: ref_31 article-title: Analysis of SARS-CoV-2 variant mutations reveals neutralization escape mechanisms and the ability to use ACE2 receptors from additional species publication-title: Immunity doi: 10.1016/j.immuni.2021.06.003 contributor: fullname: Wang – volume: 10 start-page: 19630 year: 2020 ident: ref_56 article-title: The emerging role of neutrophil extracellular traps in severe acute respiratory syndrome coronavirus 2 (COVID-19) publication-title: Sci. Rep. doi: 10.1038/s41598-020-76781-0 contributor: fullname: Arcanjo – volume: 182 start-page: 1295 year: 2020 ident: ref_19 article-title: Deep Mutational Scanning of SARS-CoV-2 Receptor Binding Domain Reveals Constraints on Folding and ACE2 Binding publication-title: Cell doi: 10.1016/j.cell.2020.08.012 contributor: fullname: Starr – volume: 81 start-page: e6 year: 2020 ident: ref_16 article-title: Neutrophil-to-lymphocyte ratio as an independent risk factor for mortality in hospitalized patients with COVID-19 publication-title: J. Infect. contributor: fullname: Liu – volume: 399 start-page: 437 year: 2022 ident: ref_50 article-title: Early assessment of the clinical severity of the SARS-CoV-2 omicron variant in South Africa: A data linkage study publication-title: Lancet doi: 10.1016/S0140-6736(22)00017-4 contributor: fullname: Wolter – volume: 3 start-page: 1049 year: 2021 ident: ref_58 article-title: GISAID’s Role in Pandemic Response publication-title: China CDC Wkly. doi: 10.46234/ccdcw2021.255 contributor: fullname: Khare – volume: 75 start-page: e1128 year: 2021 ident: ref_40 article-title: Clinical and virological features of SARS-CoV-2 variants of concern: A retrospective cohort study comparing B.1.1.7 (Alpha), B.1.315 (Beta), and B.1.617.2 (Delta) publication-title: Clin. Infect. Dis. doi: 10.1093/cid/ciab721 contributor: fullname: Ong – volume: 34 start-page: 419 year: 2021 ident: ref_47 article-title: New variants of SARS-CoV-2 publication-title: Rev. Esp. Quimioter. doi: 10.37201/req/071.2021 – volume: 182 start-page: 1419 year: 2020 ident: ref_65 article-title: Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment publication-title: Cell doi: 10.1016/j.cell.2020.08.001 contributor: fullname: Reusch – volume: 395 start-page: 565 year: 2020 ident: ref_7 article-title: Genomic characterisation and epidemiology of 2019 novel coronavirus: Implications for virus origins and receptor binding publication-title: Lancet doi: 10.1016/S0140-6736(20)30251-8 contributor: fullname: Lu – ident: ref_2 – volume: 42 start-page: 439 year: 2021 ident: ref_67 article-title: How Brazil’s President turned the country into a global epicenter of COVID-19 publication-title: J. Public Health Policy doi: 10.1057/s41271-021-00302-0 contributor: fullname: Ferrante – volume: 5 start-page: 254 year: 2020 ident: ref_22 article-title: SARS-CoV-2 spike protein: Flexibility as a new target for fighting infection publication-title: Signal Transduct. Target. Ther. doi: 10.1038/s41392-020-00369-3 contributor: fullname: Pierri – volume: 602 start-page: 300 year: 2022 ident: ref_33 article-title: Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation publication-title: Nature doi: 10.1038/s41586-021-04266-9 contributor: fullname: Saito – ident: ref_62 doi: 10.3390/v13112178 – volume: 9 start-page: 784300 year: 2021 ident: ref_26 article-title: Dynamics of SARS-CoV-2 Variants of Concern in Brazil, Early 2021 publication-title: Front. Public Health doi: 10.3389/fpubh.2021.784300 contributor: fullname: Levi – volume: 323 start-page: 1061 year: 2020 ident: ref_3 article-title: Clinical Characteristics of 138 Hospitalized Patients with 2019 Novel Coronavirus-Infected Pneumonia in Wuhan, China publication-title: JAMA doi: 10.1001/jama.2020.1585 contributor: fullname: Wang – volume: 193 start-page: E1619 year: 2021 ident: ref_45 article-title: Evaluation of the relative virulence of novel SARS-CoV-2 variants: A retrospective cohort study in Ontario, Canada publication-title: CMAJ doi: 10.1503/cmaj.211248 contributor: fullname: Fisman – volume: 327 start-page: 583 year: 2022 ident: ref_49 article-title: Characteristics and Outcomes of Hospitalized Patients in South Africa during the COVID-19 Omicron Wave Compared with Previous Waves publication-title: JAMA doi: 10.1001/jama.2021.24868 contributor: fullname: Maslo – volume: 55 start-page: 1284 year: 2022 ident: ref_66 article-title: Mass cytometry reveals a conserved immune trajectory of recovery in hospitalized COVID-19 patients publication-title: Immunity doi: 10.1016/j.immuni.2022.06.004 contributor: fullname: Burnett – volume: 2 start-page: 100166 year: 2021 ident: ref_64 article-title: A distinct innate immune signature marks progression from mild to severe COVID-19 publication-title: Cell Rep. Med. doi: 10.1016/j.xcrm.2020.100166 contributor: fullname: Chevrier – volume: 11 start-page: 3810 year: 2020 ident: ref_14 article-title: Activation and evasion of type I interferon responses by SARS-CoV-2 publication-title: Nat. Commun. doi: 10.1038/s41467-020-17665-9 contributor: fullname: Lei – volume: 12 start-page: 668507 year: 2021 ident: ref_57 article-title: Interleukin-6, CXCL10 and Infiltrating Macrophages in COVID-19-Related Cytokine Storm: Not One for All but All for One! publication-title: Front. Immunol. doi: 10.3389/fimmu.2021.668507 contributor: fullname: Coperchini – volume: 193 start-page: 407 year: 2016 ident: ref_17 article-title: Innate Lymphoid Cells Are the Predominant Source of IL-17A during the Early Pathogenesis of Acute Respiratory Distress Syndrome publication-title: Am. J. Respir. Crit. Care Med. doi: 10.1164/rccm.201410-1782OC contributor: fullname: Muir |
| SSID | ssj0000913851 |
| Score | 2.3030005 |
| Snippet | The SARS-CoV-2 P.1 variant, responsible for an outbreak in Manaus, Brazil, is distinguished by 12 amino acid differences in the S protein, potentially... |
| SourceID | doaj proquest gale crossref pubmed |
| SourceType | Open Website Aggregation Database Index Database |
| StartPage | 720 |
| SubjectTerms | Affinity Amino acids Antibodies B.1 strain Comparative analysis COVID-19 COVID-19 vaccines Cytokine storm Cytokines Disease transmission Genetic aspects Genomes Health aspects Host-virus relationships Immune response Immune system Immunogenicity Infections Infectivity Innate immunity Leukocytes (mononuclear) Leukocytes (neutrophilic) mononuclear cells Multisystem inflammatory syndrome in children Mutation Neutrophils P.1 variant Proteins SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 Structural analysis Viral diseases Viral infections Virulence (Microbiology) Viruses |
| Title | A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/38674664 https://www.proquest.com/docview/3046994645 https://search.proquest.com/docview/3047951897 https://doaj.org/article/43b891a58f0e446fbba207b73ffc3129 |
| Volume | 12 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELagEhIX1PJMKZWRkDhZmzhOHB-XpYVeODRQcbP8lBDabKW0SPyI_ufO2NmUBSQuHJM4Uex5-Bt75jMhb7izHTddxXw0kokqBma8qVlsrZJecGdMWrro5aev3fsTpMmZj_rCnLBMD5wHbiFq26nKNF0sA4Qu0VrDS2llHaOrYbJK3rdsfwmmkg9WVQ1YIpcE1xDXL9aY35ZPShrXY8VLZAYrd2ajRNr_p2v-DXCmied0nzyaECNd5j89IPfC8Jg8yGdI_nxCbpZ0dcfgTbckI3QT6dkwAJKkZ1gCEuh5zoYNIzWDp4D7aJ-4Y5F3g65m3uZclomv95ffvgeK9Se0X573bLW5YJx-MOu1oRcQYmMGTfoWOJ8f0_VT8uX05PPqI5vOWGAOXOQVk6EsjWuFtL4RwvHSQnxjnI8lSEpGAHOybaTl3FTKNSJAfGalt1X0AQIRK-pnZG_YDOEFoSCRaAPYPyAuUfmorOMBtxk7r0wQTUEW27HWl5lKQ0MIgtLRf5dOQd6hSObWSIWdbkBLPSmI_peCFOQtClSjwV7BUJqp7gB-Gqmv9FIminlAUgU52mkJhuZ2H29VQk-GPmrcWFYKt4cL8np-jG9i8toQNtepjQQg2ylZkOdZleYu1V0rkeH_8H909SV5yAFz5cSiI7IHWhRekfujvz5O5nGcFrNuARxgF3g |
| link.rule.ids | 315,782,786,866,2106,27933,27934 |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Comparative+Analysis+of+Innate+Immune+Responses+and+the+Structural+Characterization+of+Spike+from+SARS-CoV-2+Gamma+Variants+and+Subvariants&rft.jtitle=Microorganisms+%28Basel%29&rft.au=Scovino%2C+Aline+Miranda&rft.au=Dahab%2C+Elizabeth+Chen&rft.au=Diniz-Lima%2C+Israel&rft.au=de+Senna+Silveira%2C+Etiele&rft.date=2024-04-01&rft.issn=2076-2607&rft.eissn=2076-2607&rft.volume=12&rft.issue=4&rft_id=info:doi/10.3390%2Fmicroorganisms12040720&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2076-2607&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2076-2607&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2076-2607&client=summon |