Ethylene-Inhibited Jasmonic Acid Biosynthesis Promotes Mesocotyl/Coleoptile Elongation of Etiolated Rice Seedlings

• Published in: The Plant cell Vol. 29; no. 5; pp. 1053 - 1072
• Main Authors: Xiong, Qing, Ma, Biao, Lu, Xiang, Huang, Yi-Hua, He, Si-Jie, Yang, Chao, Yin, Cui-Cui, Zhao, He, Zhou, Yang, Zhang, Wan-Ke, Wang, Wen-Sheng, Li, Zhi-Kang, Chen, Shou-Yi, Zhang, Jin-Song
• Format: Journal Article
• Language: English
• Published: England American Society of Plant Biologists 01-05-2017
• Subjects: Applications programs; Aquatic plants; Biosynthesis; Chloroplasts; Cloning; Coleoptile; Cotyledon - drug effects; Cotyledon - genetics; Cotyledon - metabolism; Cyclopentanes - metabolism; Elongation; Emergence; Ethylene; Ethylenes - pharmacology; Gene expression; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - genetics; Jasmonic acid; Oryza - drug effects; Oryza - genetics; Oryza - metabolism; Oryza sativa; Oxylipins - metabolism; Phospholipase; Plant breeding; Plant Proteins - genetics; Plant Proteins - metabolism; Regulatory mechanisms (biology); Rice; Seedlings; Seedlings - drug effects; Seedlings - genetics; Seedlings - metabolism; Signal transduction; Signaling
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.16.00981

Elongation of the mesocotyl and coleoptile facilitates the emergence of rice (Oryza sativa) seedlings from soil and is affected by various genetic and environment factors. The regulatory mechanism underlying this process remains largely unclear. Here, we examined the regulation of mesocotyl and coleoptile growth by characterizing a gaoyao1 (gy1) mutant that exhibits a longer mesocotyl and longer coleoptile than its original variety of rice. GY1 was identified through map-based cloning and encodes a PLA1-type phospholipase that localizes in chloroplasts. GY1 functions at the initial step of jasmonic acid (JA) biosynthesis to repress mesocotyl and coleoptile elongation in etiolated rice seedlings. Ethylene inhibits the expression of GY1 and other genes in the JA biosynthesis pathway to reduce JA levels and enhance mesocotyl and coleoptile growth by promoting cell elongation. Genetically, GY1 acts downstream of the OsEIN2-mediated ethylene signaling pathway to regulate mesocotyl/coleoptile growth. Through analysis of the resequencing data from 3000 rice accessions, we identified a single natural variation of the GY1 gene, GY1
376T, which contributes to mesocotyl elongation in rice varieties. Our study reveals novel insights into the regulatory mechanism of mesocotyl/coleoptile elongation and should have practical applications in rice breeding programs.