Improved Immunohistochemical Method for Detecting Hypoxia Gradients in Mouse Tissues and Tumors

We describe an improved immunohistochemical procedure for detecting regions of hypoxia in normal organs and tumors in mice. The method employs a primary fluorescein-conjugated mouse monoclonal antibody directed against pimonidazole protein adducts that are created in hypoxic tissues and a secondary...

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Bibliographic Details

Published in:	The journal of histochemistry and cytochemistry Vol. 52; no. 6; pp. 837 - 839
Main Authors:	Samoszuk, Michael K, Walter, James, Mechetner, Eugene
Format:	Journal Article
Language:	English
Published:	Los Angeles, CA Histochemical Soc 01-06-2004 SAGE Publications
Subjects:	Animals Antibodies, Monoclonal Cell Hypoxia Fluorescein-5-isothiocyanate - chemistry Fluorescent Dyes - chemistry Immunohistochemistry - methods Mice Neoplasms - metabolism Nitroimidazoles - chemistry Nitroimidazoles - immunology Organ Specificity hypoxia immunohistochemistry monoclonal antibodies
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