The first report in Brazil of severe infection caused by community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA)

• Published in: Brazilian journal of medical and biological research Vol. 42; no. 8; pp. 756 - 760
• Main Authors: Rozenbaum, R, Sampaio, M G, Batista, G S, Garibaldi, A M, Terra, G M F, Souza, M J, Vieira, E N, Silva-Carvalho, M C, Teixeira, L A, Figueiredo, A M S
• Format: Journal Article
• Language: English
• Published: Brazil Associação Brasileira de Divulgação Científica 01-08-2009
• Subjects: Antimicrobial resistance; Bacterial Typing Techniques; BIOLOGY; Brazil - epidemiology; CA-MRSA; Child; Community-Acquired Infections - diagnosis; Community-Acquired Infections - microbiology; Female; Humans; MEDICINE, RESEARCH & EXPERIMENTAL; Methicillin-Resistant Staphylococcus aureus - isolation & purification; Microbial Sensitivity Tests; MRSA; PVL; S. aureus; Severity of Illness Index; Staphylococcal Infections - diagnosis; Staphylococcal Infections - epidemiology; Staphylococcal Infections - microbiology; Brazil; CA-MRSA; S. aureus; Antimicrobial resistance; MRSA; PVL
• ISSN: 0100-879X; 1414-431X; 1414-431X
• DOI: 10.1590/S0100-879X2009005000007

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emergent pathogen in Brazil. However, there are no data on the prevalence of CA-MRSA. We report here the first well-characterized case of severe life-threatening CA-MRSA infection in a child living in Rio de Janeiro city. The patient had many complications including hematogenous osteomyelitis and involvement of multiple sites requiring drainage of soft-tissue abscess, and pleural and pericardial empyema. The MRSA isolates recovered were genotyped using PFGE, SCCmec typing and multilocus sequence typing. Disk diffusion tests were performed following Clinical and Laboratory Standards Institute recommendations. In addition, the presence of Panton-Valentine leukocidin (PVL) was assessed by PCR amplification, using specific primers for lukF-pv (encoding for the F subunit of the PVL). The bacterial isolates were related to the ST30-SCCmecIV lineage (Oceania Southwest Pacific clone), a PVL producer CA-MRSA previously detected in Porto Alegre, RS, Brazil. Also, the isolates analyzed were susceptible to all non-beta-lactam antibiotics tested. The present report demonstrates that disseminated CA-MRSA disease is also occurring in Rio de Janeiro. Thus, the empirical treatment of moderate or severe infections suspected of being associated with CA-MRSA needs to be reviewed in order to allow prompt initiation of an effective therapy that also covers these microorganisms.