A new allergen family involved in pollen food-associated syndrome: Snakin/gibberellin-regulated proteins

• Published in: Journal of allergy and clinical immunology Vol. 141; no. 1; pp. 411 - 414.e4
• Main Authors: Sénéchal, Hélène, Šantrůček, Jiří, Melčová, Magdalena, Svoboda, Petr, Zídková, Jarmila, Charpin, Denis, Guilloux, Laurence, Shahali, Youcef, Selva, Marie-Ange, Couderc, Rémy, Aizawa, Tomoyasu, Poncet, Pascal
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-2018; Elsevier Limited; Elsevier
• Subjects: Allergens - chemistry; Allergens - immunology; Allergology; Amino Acid Sequence; Antigens, Plant - chemistry; Antigens, Plant - immunology; Antimicrobial agents; Cross Reactions - immunology; Cupressaceae; Food allergies; Food Hypersensitivity - diagnosis; Food Hypersensitivity - immunology; Fruits; Gibberellins - chemistry; Gibberellins - immunology; Humans; Immunoglobulin E - immunology; Immunology; Immunotherapy; Life Sciences; Mass Spectrometry; Patients; Peptides; Plant Proteins - chemistry; Plant Proteins - immunology; Pollen; Pollen - chemistry; Pollen - immunology; Proteins; Scientific imaging; Theobroma cacao
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2017.06.041

Up to now, the cross-reactive allergen at the basis of this syndrome has not yet been unraveled. Besides the 4 groups of allergens already described in the various Cupressaceae species, an as yet unidentified basic allergen of 14 kDa (BP14), overexpressed in Cupressus sempervirens pollen and different from a lipid transfer protein, was found to sensitize 37% of cypress pollen allergic patients (CPAPs) in Southern France.3 The BP14 IgE epitopes are not related to cross-reactive carbohydrate determinants and are heat resistant but destroyed under reducing conditions. Because Pru p 7 shares more than 80% sequence identity with snakin-1 and more than 95% with other fruit GRP allergens, BP14 should be considered as the cross-reactive allergen in the 2 documented PFAS involving peach and/or citrus.1 Preliminary experiments showed that patients allergic to cypress pollen and citrus expressed IgE against cypress pollen BP14 cross-reactive with a cationic 12-kDa allergen from grapefruit.9 Therefore, snakin/GRP may represent a new diagnostic marker of PFAS in addition to other allergens such as Bet v 1-like proteins involved in the well-studied birch/apple syndrome.10 Whether specific allergenic immunotherapy using a BP14-containing cypress pollen extract can reduce the food-related symptoms remains unknown for the moment and would deserve more investigations. [...]proteins were digested for 3 hours at 37°C in 50 mM NH4HCO3 with trypsin (concentration 12.5 μg/mL, sequencing grade, Pierce, Rockford, Ill). IgE reactivity on Immunosorbent for depletion Snakin-1 Cypress pollen extract 5 Snakin-1 + + BP14 in basic fraction − + 12 Snakin-1 + + BP14 in basic fraction − + Snakin-1 +∗ +† 14 BP14 in total extract − + BP14 in basic fraction −∗ +† 16 Snakin-1 ND ND BP14 in basic fraction − + 19 Snakin-1 + + BP14 in basic fraction − + 1 D. Charpin, C. Pichot, J. Belmonte, J.P. Sutra, J. Zidkova, P. Chanez, Cypress pollinosis: from tree to clinic, Clin Rev Allergy Immunol, April 11, 2017, Epub ahead of print 2 C. Truong van ut, F. Trébuchon, J. Birnbaum, M. Agell, R. Navarro-Rouimi, G. Gentile, Knowledge and behavior of patients with allergic rhinitis during a consultation with primary care in general practitioner, Rev Fr Allerg, Vol. 52, 2012, 429-436 3 Y. Shahali, P. Nicaise, A. Brazdova, D. Charpin, E. Scala, A. Mari, Complementarity between microarray and immunoblot for the comparative evaluation of IgE repertoire of French and Italian Cypress pollen allergic patients, Folia Biol (Praha), Vol. 60, 2014, 192-201 4 R. Kuddus, F. Rumi, M. Tsutsumi, R. Takahashi, M. Yamano, M. Kamiya, Pichia pastoris, Protein Expr Purif, Vol. 122, 2016, 15-22 5 V. Nahirnak, N.I. Almasia, H.E. Hopp, C. Vazquez-Rovere, Plant Signal Behav, Vol. 7, 2012, 1004-1008 6 M. Mahlapuu, J. Hakansson, L. Ringstad, C. Bjorn, Antimicrobial peptides: an emerging category of therapeutic agents, Front Cell Infect Microbiol, Vol. 6, 2016, 194 7 L. Tuppo, C. Alessandri, D. Pomponi, D. Picone, M. Tamburrini, R. Ferrara, Peamaclein-a new peach allergenic protein: similarities, differences and misleading features compared to Pru p 3, Clin Exp Allergy, Vol. 43, 2013, 128-140 8 L. Tuppo, C. Alessandri, M.S. Pasquariello, M. Petriccione, I. Giangrieco, M. Tamburrini, Pomegranate cultivars: identification of the new IgE-binding protein pommaclein and analysis of antioxidant variability, J Agric Food Chem, Vol. 65, 2017, 2702-2710 9 S. Martinez, M. Gouitaa, M. Alter, C. Longè, A. Brazdova, R. Couderc, June 6, 2015, 34th EAACI meeting, Barcelona, Spain 10 T. Werfel, R. Asero, B. Ballmer-Weber, K. Beyer, E. Enrique, A. Knulst, Position paper of the EAACI: food allergy due to immunological cross-reactions with common inhalant allergens, Allergy, Vol. 70, 2015, 1079-1090 11 C. Inuo, Y. Kondo, K. Tanaka, Y. Nakajima, T. Nomura, H. Ando, Japanese cedar pollen-based subcutaneous immunotherapy decreases tomato fruit-specific basophil activation, Int Arch Allergy Immunol, Vol. 167, 2015, 137-145 12 N. Inomata, M. Miyakawa, M. Aihara, High prevalence of sensitization to gibberellin-regulated protein (peamaclein) in fruit allergies with negative immunoglobulin E reactivity to Bet v. 1 homologs and profilin: clinical pattern, causative fruits and cofactor effect of gibberellin-regulated protein allergy, J Dermatol, March 22, 2017, Epub ahead of print