Aptamer conjugated paclitaxel and magnetic fluid loaded fluorescently tagged PLGA nanoparticles for targeted cancer therapy

• Published in: Journal of magnetism and magnetic materials Vol. 344; pp. 116 - 123
• Main Authors: Aravind, Athulya, Nair, Remya, Raveendran, Sreejith, Veeranarayanan, Srivani, Nagaoka, Yutaka, Fukuda, Takahiro, Hasumura, Takahashi, Morimoto, Hisao, Yoshida, Yasuhiko, Maekawa, Toru, Sakthi Kumar, D.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-10-2013; Elsevier
• Subjects: AS1411 aptamer; Binding; Biological and medical sciences; Cancer; Cancer therapy; Drugs; Magnetic fluid; Magnetic fluids; Medical sciences; Nanodrug delivery; Nanoparticles; Nanostructure; Paclitaxel; PLGA Nanoparticles; Therapy; Treatment. General aspects; Tumors; Nanodrug delivery; AS1411 aptamer; Magnetic fluid; PLGA Nanoparticles; Cancer therapy; Paclitaxel; Antineoplastic agent; Ferromagnetic fluid; Drug delivery systems; Magnetization; Organic dye; Nanoparticle; Confocal microscopy; In vitro; Conjugated polymer; Magnetic particles; Functionalization; Image contrast
• ISSN: 0304-8853
• DOI: 10.1016/j.jmmm.2013.05.036

Controlled and targeted drug delivery is an essential criterion in cancer therapy to reduce the side effects caused by non-specific drug release and toxicity. Targeted chemotherapy, sustained drug release and optical imaging have been achieved using a multifunctional nanocarrier constructed from poly (D, l-lactide-co-glycolide) nanoparticles (PLGA NPs), an anticancer drug paclitaxel (PTX), a fluorescent dye Nile red (NR), magnetic fluid (MF) and aptamers (Apt, AS1411, anti-nucleolin aptamer). The magnetic fluid and paclitaxel loaded fluorescently labeled PLGA NPs (MF-PTX-NR-PLGA NPs) were synthesized by a single-emulsion technique/solvent evaporation method using a chemical cross linker bis (sulfosuccinimidyl) suberate (BS3) to enable binding of aptamer on to the surface of the nanoparticles. Targeting aptamers were then introduced to the particles through the reaction with the cross linker to target the nucleolin receptors over expressed on the cancer cell surface. Specific binding and uptake of the aptamer conjugated magnetic fluid loaded fluorescently tagged PLGA NPs (Apt-MF-NR-PLGA NPs) to the target cancer cells induced by aptamers was observed using confocal microscopy. Cytotoxicity assay conducted in two cell lines (L929 and MCF-7) confirmed that targeted MCF-7 cancer cells were killed while control cells were unharmed. In addition, aptamer mediated delivery resulting in enhanced binding and uptake to the target cancer cells exhibited increased therapeutic effect of the drug. Moreover, these aptamer conjugated magnetic polymer vehicles apart from actively transporting drugs into specifically targeted tumor regions can also be used to induce hyperthermia or for facilitating magnetic guiding of particles to the tumor regions.
•Aptamer escorted, theranostic biodegradable PLGA carriers were developed.•Can target cancer cells, control drug release, image and magnetically guide.•Highly specific to the targeted cancer cells thus delivering the drugs accurately.•The magnetic field responsive NPs facilitate cancer therapy with less side effects.•Effective therapy is achieved by synergetic effect of aptamers and magnetic field.