Ikaros Confers Early Temporal Competence to Mouse Retinal Progenitor Cells

• Published in: Neuron (Cambridge, Mass.) Vol. 60; no. 1; pp. 26 - 39
• Main Authors: Elliott, Jimmy, Jolicoeur, Christine, Ramamurthy, Vasanth, Cayouette, Michel
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09-10-2008; Elsevier Limited
• Subjects: Animals; Cell Lineage - genetics; Cell Lineage - physiology; CELLBIO; Deoxyribonucleic acid; DEVBIO; DNA; Drosophila; Experiments; Humans; Ikaros Transcription Factor - biosynthesis; Ikaros Transcription Factor - genetics; Ikaros Transcription Factor - physiology; Insects; Medical research; Mice; Mice, Inbred C57BL; Mice, Knockout; MOLNEURO; Neurons; Photoreceptors; Rats; Rats, Sprague-Dawley; Retina; Retina - cytology; Retina - embryology; Retina - physiology; Stem Cells - cytology; Stem Cells - physiology; Transcription factors; CELLBIO; DEVBIO; MOLNEURO
• ISSN: 0896-6273; 1097-4199
• DOI: 10.1016/j.neuron.2008.08.008

In the developing mouse retina, multipotent retinal progenitor cells (RPCs) give rise to specific retinal cell types at different times, but the molecular mechanisms regulating how RPCs change over time remain unclear. In the
Drosophila neuroblast lineage, the zinc finger transcription factor Hunchback (Hb) is both necessary and sufficient to specify early-born neuronal identity. We show here that Ikaros, a mouse ortholog of Hb, is expressed in all early embryonic RPCs, which then give rise to Ikaros-negative RPCs at later stages in the lineage. Remarkably, misexpression of Ikaros in late RPCs is sufficient to confer competence to generate early-born neurons. Conversely, Ikaros mutant mice have reduced numbers of early-born cell types, whereas late-born cell types are not affected. These results suggest a model in which Ikaros expression is both necessary and sufficient to confer early temporal competence to RPCs and raise the possibility that a similar strategy might be used to control the sequential order of cell birth in other parts of the nervous system.