Interleukin-10 receptor expression in human dental pulp cells in response to lipopolysaccharide from Prevotella intermedia

• Published in: Journal of endodontics Vol. 29; no. 1; p. 48
• Main Authors: Tokuda, Masayuki, Nagaoka, Shigetaka, Torii, Mitsuo
• Format: Journal Article
• Language: English
• Published: United States 01-01-2003
• Subjects: Adolescent; Cells, Cultured; Dental Pulp - cytology; Dental Pulp - drug effects; Dental Pulp - metabolism; Fibroblasts - drug effects; Fibroblasts - metabolism; Gene Expression; Humans; Interleukin-10 - metabolism; Lipopolysaccharides - pharmacology; Male; Prevotella intermedia - chemistry; Receptors, Interleukin - antagonists & inhibitors; Receptors, Interleukin - biosynthesis; Receptors, Interleukin - genetics; Receptors, Interleukin-10; Recombinant Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; Skin - cytology; Skin - metabolism; U937 Cells - metabolism
• ISSN: 0099-2399
• DOI: 10.1097/00004770-200301000-00013

Interleukin-10 receptor (IL-10R) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. After exposure to lipopolysaccharide (LPS) from Prevotella intermedia, the IL-10R mRNA levels increased after 4 h, peaked at 7 h, and dropped back to the unstimulated level at 24 h. Maximal production of the IL-10R protein in dental pulp fibroblast cultures was detected by Western blot analysis after 12 h of LPS stimulation. In contrast, the human skin fibroblast (SF-MA) and human monocyte (U937) cell lines expressed IL-10R mRNA. Anti-CD14 antibodies inhibited P. intermedia LPS-induced IL-10R mRNA expression. These results indicate that P. intermedia LPS induces IL-10R gene expression in human, dental pulp fibroblasts in vitro.