Cytokine mapping in cerebrospinal fluid and blood in multiple sclerosis patients without oligoclonal bands

• Published in: Multiple sclerosis Vol. 18; no. 5; pp. 669 - 673
• Main Authors: Vrethem, M, Kvarnström, M, Stenstam, J, Cassel, P, Gustafsson, M, Landtblom, AM, Ernerudh, J
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01-05-2012; Sage Publications; Sage Publications Ltd
• Subjects: Adult; Aged; Biological and medical sciences; Biomarkers - blood; Biomarkers - cerebrospinal fluid; Blood; Case-Control Studies; Cells, Cultured; Cerebrospinal fluid; Chi-Square Distribution; Cytokines; Cytokines - blood; Cytokines - cerebrospinal fluid; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Female; gamma -Interferon; Gene mapping; Granulocyte-macrophage colony-stimulating factor; Humans; Immune response; Inflammation; Inflammation Mediators - blood; Inflammation Mediators - cerebrospinal fluid; Interleukin 1; Interleukin 10; Interleukin 12; Interleukin 13; Interleukin 15; Interleukin 17; Interleukin 6; Interleukin 8; Leukocytes (mononuclear); Leukocytes, Mononuclear - immunology; Male; Medical sciences; MEDICIN; MEDICINE; Middle Aged; Multiple sclerosis; Multiple Sclerosis - blood; Multiple Sclerosis - cerebrospinal fluid; Multiple Sclerosis - classification; Multiple Sclerosis - diagnosis; Multiple Sclerosis - immunology; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis; Neurology; Oligoclonal Bands - cerebrospinal fluid; Regression Analysis; Sweden; Tumor necrosis factor; vascular cell adhesion molecule 1; Young Adult; Sweden; cytokines; multiple sclerosis; cerebrospinal fluid; oligoclonal bands; Human; Cartography; Nervous system diseases; Multiple sclerosis; Central nervous system disease; Cytokine; Degenerative disease; Cerebrospinal fluid; Inflammatory disease
• ISSN: 1352-4585; 1477-0970; 1477-0970
• DOI: 10.1177/1352458511424903

Objective: Since there are clinical and genetic differences between MS patients with intrathecal oligoclonal bands (OCB+) in the cerebrospinal fluid (CSF) compared with those without (OCB−), the aim was to find out if OCB− patients showed a different pattern of cytokine immune activation compared with OCB+ patients.
Methods: The study included 25 MS patients (10 OCB− and 15 OCB+) and 13 controls. A panel of cytokines was measured; IL-1β, IL-6, IL-8/CXCL8, IL-10, TNF and GM-CSF in serum, CSF and in supernatants from polyclonally stimulated blood mononuclear cells, where also levels of IL-12p40, IL-13, IL-15, IL-17 and IFN-γ were measured. The concentrations of soluble (s) VCAM-1 and sCD14 were measured in serum and CSF.
Results: In general, there were no extensive differences in cytokine concentrations between the OCB− and OCB+ groups.
Conclusion: OCB− MS patients do not seem to constitute a separate entity concerning inflammatory parameters measured as cytokine concentrations in CSF and blood.