Substance P mRNA expression during zebrafish development: influence of mu opioid receptor and cocaine

• Published in: Neuroscience Vol. 242; pp. 53 - 68
• Main Authors: López-Bellido, Roger, Barreto-Valer, Katherine, Rodríguez, Raquel Emilia
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 09-07-2013; Elsevier
• Subjects: Animals; Biological and medical sciences; Central Nervous System - metabolism; cocaine; Cocaine - pharmacology; development; Embryonic Development - drug effects; Embryonic Development - genetics; Embryonic Development - physiology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental - drug effects; Gene Expression Regulation, Developmental - genetics; Gene Knockdown Techniques; Humans; Medical sciences; Myocardium - metabolism; Neurology; Neuropharmacology; opioid receptors; Pharmacology. Drug treatments; Phylogeny; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease); Psychology. Psychoanalysis. Psychiatry; Psychopharmacology; Receptors, Opioid, delta - genetics; Receptors, Opioid, mu - genetics; Receptors, Opioid, mu - physiology; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Somites - metabolism; substance P; Substance P - genetics; TAC1; Vertebrates: nervous system and sense organs; Zebrafish; PPT-A; MHB; PFA; preprotachykinin-A; midbrain–hindbrain boundary; EKs; Maximum Likelihood; Cq; MOs; NAc; Tris-buffered saline; ethylenediaminetetraacetic acid; paraformaldehyde; substance P; ML; PBS; development; NKB; TBS; NKA; nucleus accumbens; endokinins; NPγ; hydrochloride; hour post-fertilisation; ventral tegmental area; CRH; morpholino oligonucleotides; SEM; standard error of the mean; NJ; Molecular Evolutionary Genetic Analysis; phosphate saline buffer; opioid receptors; ORF; VTA; cocaine; HK-1; hemokinin 1; open reading frame; tachykinin; HCl; analysis of variance; ANOVA; MEGA; TAC1; SP; hpf; common cardinal vein; neuropeptide γ; corticotropin-releasing hormone; EDTA; cycle quantification; CCV; Neighbour-Joining; TK; neurokinin A; neurokinin B; CNS stimulant; Psychotropic; Substance P; Opioid receptor; μ Opioid receptor; Gene expression; Neuropeptide; Vertebrata; Brachydanio rerio; Pisces; Development; Drug of abuse; Cocaine; Tachykinin
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.03.022

Highlights • The TAC1- tachykinin precursor gene that encodes substance P is present in zebrafish. • Substance P (SP) mRNA increases progressively during zebrafish development. • SP is expressed in the central nervous system and peripheral areas in zebrafish. • Cocaine altered the expression levels of SP in zebrafish embryos. • SP expression is affected by the knockdown of μ-opioid receptor.